Cellular uptake of adamantyl conjugated peptide nucleic acids

被引：64

作者：

Ljungstrom, T ^{[1
]}

Knudsen, H ^{[1
]}

Nielsen, PE ^{[1
]}

机构：

[1] Univ Copenhagen, Panum Inst, Ctr Biomol Recognit, Dept Med Biochem & Genet,Biochem Lab B, DK-2200 Copenhagen N, Denmark

来源：

BIOCONJUGATE CHEMISTRY | 1999年 / 10卷 / 06期

关键词：

D O I：

10.1021/bc990053+

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Peptide nucleic acids (PNA) (15-mers) conjugated to adamantyl acetic acid and labeled with fluorescein have been prepared, and their (liposome mediated) uptake in human cells in culture (HeLa, IMR-90 and MDA-MB-453) has been studied by confocal fluorescence microscopy. It is found that adamantyl-PNAs show greatly improved (endosomal) cellular uptake, but that this uptake is dependent on the cell line. Cellular uptake of such lipophilic PNAs is further mediated by cationic liposomes, and in some cases, the intracellular localization is diffuse cytoplasmic or nuclear, again cell-type dependent. The results show that this simple PNA modification can indeed greatly improve cellular uptake, but the effect appears strongly cell-type as well as PNA-sequence dependent.

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收藏

页码：965 / 972

页数：8

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