Growth mechanism of the (110) face of tetragonal lysozyme crystals

被引:46
作者
Nadarajah, A [1 ]
Li, MR [1 ]
Pusey, ML [1 ]
机构
[1] UNIV ALABAMA,NASA,GEORGE C MARSHALL SPACE FLIGHT CTR,BIOPHYS BRANCH ES76,HUNTSVILLE,AL 35899
来源
ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY | 1997年 / 53卷
关键词
D O I
10.1107/S0907444997003041
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
The measured macroscopic growth rates of the (110) face of tetragonal lysozyme show an unexpectedly complex dependence on the supersaturation. In earlier studies it has been shown that an aggregate growth unit could account for experimental growth-rate trends. In particular molecular packing and interactions in the growth of the crystal were favored by completion of the helices along the 4(3) axes. In this study the molecular orientations of the possible growth units and the molecular growth mechanism were identified. This indicated that growth was a two-step process: aggregate growth units corresponding to the 4(3) helix are first formed in the bulk solution by stronger intermolecular bonds and then attached to the crystal face by weaker bonds. A more comprehensive analysis of the measured (110) growth rates was also undertaken. They were compared with the predicted growth rates from several dislocation and two-dimensional nucleation growth models, employing tetramer and octamer growth units in polydisperse solutions and monomer units in monodisperse solutions. The calculations consistently showed that the measured growth rates followed the expected model relations with octamer growth units, in agreement with the predictions from the molecular level analyses.
引用
收藏
页码:524 / 534
页数:11
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