A gene of the major facilitator superfamily encodes a transporter for enterobactin (Enb1p) in Saccharomyces cerevisiae

被引:89
作者
Heymann, P
Ernst, JF
Winkelmann, G [1 ]
机构
[1] Univ Tubingen, Dept Microbiol & Biotechnol, Tubingen, Germany
[2] Univ Dusseldorf, Inst Microbiol, D-4000 Dusseldorf, Germany
关键词
major facilitator superfamily; iron transport; siderophores; enterobactin; Saccharomyces cerevisiae;
D O I
10.1023/A:1009250017785
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
While in fungi iron transport via hydroxamate siderophores has been amply proven, iron transport via enterobactin is largely unknown. Enterobactin is a catecholate-type siderophore produced by several enterobacterial genera grown in severe iron deprivation. By using the KanMX disruption module in vector pUG6 in a fet3 Delta background of Saccharomyces cerevisiae we were able to disrupt the gene YOL158c Sce of the major facilitator super family (MFS) which has been previously described as a gene encoding a membrane transporter of unknown function. Contrary to the parental strain, the disruptant was unable to utilize ferric enterobactin in growth promotion tests and in transport assays using (5)5Fe-enterobactin. All other siderophore transport properties remained unaffected. The results are evidence that in S. cerevisiae the YOL158c Sce gene of the major facilitator super family, now designated ENB1, encodes a transporter protein (Enb1p), which specifically recognizes and transports enterobactin.
引用
收藏
页码:65 / 72
页数:8
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