The effect of heparin on the gellation of Pluronic F-127 hydrogel

The interaction between heparin and Pluronic polymer was characterized by the sol-gel transition temperature and the theological properties of the heparin-Pluronic hydrogels. The decrease of the transition temperature was consistently shown in all media (distilled water, phosphate buffer, and PBS) as the added amount of heparin increased. In contrast, the addition of PEG produced the increase of the transition temperature. The order of phase transition with increasing temperature (heparin-Pluronic > Pluronic > PEG-Pluronic) obtained from the change in the storage modulus of the Pluronic solutions well coincided with the results obtained from the inverting vial method; a plateau of storage modulus in frequency sweep was developed at the lowest temperature for the heparin-Pluronic hydrogel, and the plateau modulus of the heparin-Pluronic hydrogel was higher than that of the pure Pluronic hydrogel near the sol-gel transition boundary. These results demonstrate that the interaction between heparin and Pluronic polymer exists, which promotes the Pluronic micelle-micelle association. (c) 2005 Elsevier B.V. All rights reserved.