Multiple spontaneous rhythmic activity patterns generated by the embryonic mouse spinal cord occur within a specific developmental time window

被引:61
作者
Yvert, B
Branchereau, P
Meyrand, P
机构
[1] CNRS, Lab Neurobiol Res, UMR 5816, F-33405 Talence, France
[2] Univ Bordeaux 1, F-33405 Talence, France
关键词
D O I
10.1152/jn.01095.2003
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Spontaneous rhythmic activity is a ubiquitous phenomenon in developing neural networks and is assumed to play an important role in the elaboration of mature circuitry. Here we describe the day-by-day evolution of spontaneous activity in the embryonic mouse spinal cord and show that, at a specific developmental stage, 2 distinct rhythms coexist. On embryonic days E12.5 and E13.5, we observed a single type of regularly recurring short spike-episodes synchronized across cervical, thoracic, and lumbar levels. By E14.5, in addition to this motor rhythm, another type of spontaneous synchronous activity appeared, characterized by much longer lasting episodes separated by longer time intervals. On E15.5, these long episodes disappeared. Short episodes were less numerous and more irregular except at the cervical level where a rhythm was occasionally observed. By E16.5, this cervical rhythm became more robust, whereas the lumbar level fell almost silent. Surprisingly, at E17.5, spontaneous activity resumed at caudal levels, now characterized by numerous erratic short episodes. A striking ontogenetic feature of spontaneous activity was the occurrence of long episodes only at E14.5. Although concomitant at all levels of the spinal cord, long episodes displayed different patterns along the spinal cord, with tonic firing at the thoracic level and rhythmic discharge with occasional sequences of left/right alternation at the lumbar level. Thus at E14.5, the originally synchronized network has started to segregate into more specialized subnetworks. In conclusion, this work suggests that ongoing spontaneous rhythms do not follow a smooth evolution during maturation, but rather undergo profound changes at very specific stages.
引用
收藏
页码:2101 / 2109
页数:9
相关论文
共 31 条
[21]   Spontaneous motoneuronal activity mediated by glycine and GABA in the spinal cord of rat fetuses in vitro [J].
Nishimaru, H ;
Iizuka, M ;
Ozaki, S ;
Kudo, N .
JOURNAL OF PHYSIOLOGY-LONDON, 1996, 497 (01) :131-143
[22]   The origin of spontaneous activity in developing networks of the vertebrate nervous system [J].
O'Donovan, MJ .
CURRENT OPINION IN NEUROBIOLOGY, 1999, 9 (01) :94-104
[23]  
O'Donovan MJ, 1998, J NEUROBIOL, V37, P131, DOI 10.1002/(SICI)1097-4695(199810)37:1<131::AID-NEU10>3.0.CO
[24]  
2-H
[25]   Brain waves and brain wiring: The role of endogenous and sensory-driven neural activity in development [J].
Penn, AA ;
Shatz, CJ .
PEDIATRIC RESEARCH, 1999, 45 (04) :447-458
[26]   PRENATAL AND POSTNATAL-DEVELOPMENT OF NORADRENERGIC PROJECTIONS TO THE RAT SPINAL-CORD - AN IMMUNOCYTOCHEMICAL STUDY [J].
RAJAOFETRA, N ;
POULAT, P ;
MARLIER, L ;
GEFFARD, M ;
PRIVAT, A .
DEVELOPMENTAL BRAIN RESEARCH, 1992, 67 (02) :237-246
[27]   Ontogeny of rhythmic motor patterns generated in the embryonic rat spinal cord [J].
Ren, J ;
Greer, JJ .
JOURNAL OF NEUROPHYSIOLOGY, 2003, 89 (03) :1187-1195
[28]   Emergence of order in visual system development [J].
Shatz, CJ .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1996, 93 (02) :602-608
[29]  
Tabak J, 2000, J NEUROSCI, V20, P3041
[30]   The role of activity-dependent network depression in the expression and self-regulation of spontaneous activity in the developing spinal cord [J].
Tabak, J ;
Rinzel, J ;
O'Donovan, MJ .
JOURNAL OF NEUROSCIENCE, 2001, 21 (22) :8966-8978