Enzymatically prepared RNAi libraries

被引：52

作者：

Buchholz, Frank ^{[1
]}

Kittler, Ralf ^{[1
]}

Slabicki, Mikolaj ^{[1
]}

Theis, Mirko ^{[1
]}

机构：

[1] Max Planck Inst Mol Cell Biol & Genet, D-01307 Dresden, Germany

来源：

NATURE METHODS | 2006年 / 3卷 / 09期

关键词：

D O I：

10.1038/NMETH912

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Large-scale RNA interference (RNAi) screens in mammalian cells have mainly used synthetic small interfering RNA (siRNA) or short hairpin RNA (shRNA) libraries. The RNAi triggers for both of these approaches were designed with algorithm-based predictions to identify single sequences for mRNA knockdown. Alternatives to these approaches have recently been developed using enzymatic methods. Here we describe the concepts of enzymatically prepared shRNA and siRNA libraries, and discuss their strengths and limitations.

引用

页码：696 / 700

页数：5

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