Expression of a sterol carrier protein-x gene in the Yellow fever mosquito, Aedes aegypti

被引：25

作者：

Lan, Q ^{[1
]}

Wessely, V ^{[1
]}

机构：

[1] Univ Wisconsin, Dept Entomol, Madison, WI 53706 USA

来源：

INSECT MOLECULAR BIOLOGY | 2004年 / 13卷 / 05期

关键词：

juvenile hormone; 20-hydroxyecdysone; post-translation modification; cholesterol; lipid metabolism;

D O I：

10.1111/j.0962-1075.2004.00510.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The sterol carrier protein-x (SCP-x), a peroxisomal thiolase/nonspecific lipid binding protein, was characterized in the yellow fever mosquito, Aedes aegypti. The Aedes aegypti SCP-x (AeSCP-x) has 83% and 75% similarities to Drosophila and mammalian SCP-x, respectively. However, the AeSCP-x gene did not produce multiple transcripts, which is characteristic of the vertebrate SCP-x gene. Levels of AeSCP-x transcription were higher in larvae and pupae. Gut tissue showed the highest level of AeSCP-x mRNA in larvae. In adults, low levels of AeSCP-x transcription were detected in both sexes. Polyclonal antibodies against the sterol carrier protein-2 (SCP-2) domain of AeSCP-x detected two proteins of 62 kDa and 13 kDa. The results indicate that AeSCP-x is proteolytically cleaved after translation to produce a smaller protein that contains only the SCP-2 domain, which is similar to post-translational modification of the vertebrate's SCP-x to produce multiple products.

引用

页码：519 / 529

页数：11

共 37 条

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