Melatonin synthesis in rice seedlings in vivo is enhanced at high temperatures and under dark conditions due to increased serotonin N-acetyltransferase and N-acetylserotonin methyltransferase activities

被引:187
作者
Byeon, Yeong [1 ]
Back, Kyoungwhan [1 ]
机构
[1] Chonnam Natl Univ, Dept Biotechnol, Interdisciplinary Program Bioenergy & Biomat, Bioenergy Res Ctr, Kwangju 500757, South Korea
基金
新加坡国家研究基金会;
关键词
daily melatonin rhythm; high temperature; melatonin; N-acetylserotonin O-methyltransferase; rice seedlings; serotonin N-acetyltransferase; GROWTH-STIMULATING COMPOUND; TRANSGENIC RICE; PLANTS; BIOSYNTHESIS; SENESCENCE; STRESS; LIGHT; GERMINATION; ANTIOXIDANT; MECHANISMS;
D O I
10.1111/jpi.12111
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Temperature and light are important environmental factors for plant growth and development. The final two enzymes in the melatonin synthesis pathway in plants are serotonin N-acetyltransferase (SNAT) and N-acetylserotonin methyltransferase (ASMT), which have thermophilic characteristics. Thus, the effects of temperature and light on melatonin synthesis in rice seedlings were investigated. Here, we demonstrated that melatonin levels increased as temperature increased when rice seedlings were exposed to various temperatures for 1hr. Moreover, the relative melatonin levels were higher in the dark. For example, exposure of rice seedlings to 1-hr darkness at 55 degrees C resulted in a melatonin yield of 4.9ng/g fresh weight (fw), compared with 2.95ng/g fw under light conditions. Temperature-dependent melatonin synthesis was closely associated with an increase in both SNAT and ASMT activities, but not with transcript levels of melatonin biosynthetic genes. The daily melatonin levels in field-grown rice plants were unaffected as the positive effect of the relatively high temperature during the day was counteracted by the negative effect of the high light. The opposite effect occurred during the night, in which the positive effect of darkness on melatonin synthesis was counteracted by the negative effect of a low temperature.
引用
收藏
页码:189 / 195
页数:7
相关论文
共 55 条
[1]   Melatonin in Glycyrrhiza uralensis:: response of plant roots to spectral quality of light and UV-B radiation [J].
Afreen, F. ;
Zobayed, S. M. A. ;
Kozai, T. .
JOURNAL OF PINEAL RESEARCH, 2006, 41 (02) :108-115
[2]   Growth conditions determine different melatonin levels in Lupinus albus L. [J].
Arnao, Marino B. ;
Hernandez-Ruiz, Josefa .
JOURNAL OF PINEAL RESEARCH, 2013, 55 (02) :149-155
[3]   Chemical stress by different agents affects the melatonin content of barley roots [J].
Arnao, Marino B. ;
Hernandez-Ruiz, Josefa .
JOURNAL OF PINEAL RESEARCH, 2009, 46 (03) :295-299
[4]   Assessment of Different Sample Processing Procedures Applied to the Determination of Melatonin in Plants [J].
Arnao, Marino B. ;
Hernandez-Ruiz, Josefa .
PHYTOCHEMICAL ANALYSIS, 2009, 20 (01) :14-18
[5]   Growth conditions influence the melatonin content of tomato plants [J].
Banon Arnao, Marino ;
Hernandez-Ruiz, Josefa .
FOOD CHEMISTRY, 2013, 138 (2-3) :1212-1214
[6]   Melatonin levels, determined by LC-ESI-MS/MS, fluctuate during the day/night cycle in Vitis vinifera cv Malbec: evidence of its antioxidant role in fruits [J].
Boccalandro, Hernan E. ;
Gonzalez, Carina V. ;
Wunderlin, Daniel A. ;
Silva, Maria F. .
JOURNAL OF PINEAL RESEARCH, 2011, 51 (02) :226-232
[7]   Cellular localization and kinetics of the rice melatonin biosynthetic enzymes SNAT and ASMT [J].
Byeon, Yeong ;
Lee, Hyoung Yool ;
Lee, Kyungjin ;
Park, Sangkyu ;
Back, Kyoungwhan .
JOURNAL OF PINEAL RESEARCH, 2014, 56 (01) :107-114
[8]   Molecular cloning and functional analysis of serotonin N-acetyltransferase from the cyanobacterium Synechocystis sp PCC 6803 [J].
Byeon, Yeong ;
Lee, Kyungjin ;
Park, Youn-Il ;
Park, Sangkyu ;
Back, Kyoungwhan .
JOURNAL OF PINEAL RESEARCH, 2013, 55 (04) :371-376
[9]   Microarray analysis of genes differentially expressed in melatonin-rich transgenic rice expressing a sheep serotonin N-acetyltransferase [J].
Byeon, Yeong ;
Park, Sangkyu ;
Kim, Young Soon ;
Back, Kyoungwhan .
JOURNAL OF PINEAL RESEARCH, 2013, 55 (04) :357-363
[10]   Light-regulated melatonin biosynthesis in rice during the senescence process in detached leaves [J].
Byeon, Yeong ;
Park, Sangkyu ;
Kim, Young-Soon ;
Park, Don-Hee ;
Lee, Sungbeom ;
Back, Kyoungwhan .
JOURNAL OF PINEAL RESEARCH, 2012, 53 (01) :107-111