Front-End Electron Transfer Dissociation: A New Ionization Source

被引：58

作者：

Earley, Lee ^{[3
]}

Anderson, Lissa C. ^{[1
]}

Bai, Dina L. ^{[1
]}

Mullen, Christopher ^{[3
]}

Syka, John E. P. ^{[3
]}

English, A. Michelle ^{[1
]}

Dunyach, Jean-Jacques ^{[3
]}

Stafford, George C., Jr. ^{[3
]}

Shabanowitz, Jeffrey ^{[1
]}

Hunt, Donald F. ^{[1
,2
]}

Compton, Philip D. ^{[1
]}

机构：

[1] Univ Virginia, Dept Chem, Charlottesville, VA 22903 USA

[2] Univ Virginia, Dept Pathol, Charlottesville, VA 22903 USA

[3] Thermo Fisher Sci, San Jose, CA 95134 USA

来源：

ANALYTICAL CHEMISTRY | 2013年 / 85卷 / 17期

关键词：

COLLISION-INDUCED DISSOCIATION; ORBITRAP MASS-SPECTROMETER;

D O I：

10.1021/ac401783f

中图分类号：

O65 [分析化学];

学科分类号：

070302 [分析化学];

摘要：

Electron transfer dissociation (ETD), a technique that provides efficient fragmentation while depositing little energy into vibrational modes, has been widely integrated into proteomics workflows. Current implementations of this technique, as well as other ion-ion reactions like proton transfer, involve sophisticated hardware, lack robustness, and place severe design limitations on the instruments to which they are attached. Described herein is a novel, electrical discharge-based reagent ion source that is located in the first differentially pumped region of the mass spectrometer. The reagent source was found to produce intense reagent ion signals over extended periods of time while having no measurable impact on precursor ion signal. Further, the source is simple to construct and enables implementation of ETD on any instrument without modification to footprint. Finally, in the context of hybrid mass spectrometers, relocation of the reagent ion source to the front of the mass spectrometer enables new approaches to gas phase interrogation of intact proteins.

引用

页码：8385 / 8390

页数：6

共 10 条

[1]

Characterization of glycopeptides by combining collision-induced dissociation and electron-transfer dissociation mass spectrometry data [J].