Immunoassay readout method using extrinsic Raman labels adsorbed on immunogold colloids

被引:430
作者
Ni, J
Lipert, RJ
Dawson, GB
Porter, MD [1 ]
机构
[1] US DOE, Ames Lab, Microanalyt Instrumentat Ctr, Ames, IA 50011 USA
[2] Iowa State Univ Sci & Technol, Dept Chem, Ames, IA 50011 USA
关键词
D O I
10.1021/ac990616a
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
An immunoassay readout method based on surface-enhanced Raman scattering (SERS) is described. The method exploits the SERS-derived signal from reporter molecules that are coimmobilized with biospecific species on gold colloids. This concept is demonstrated in a dual-analyte sandwich assay, in which two different antibodies covalently bound to a solid substrate specifically capture two different antigens from an aqueous sample. The captured antigens in turn bind selectively to their corresponding detection antibodies. The detection antibodies are conjugated with gold colloids that are labeled with different Raman reporter molecules, which serve as extrinsic labels for each type of antibody. The presence of a specific antigen is established by the characteristic SERS spectrum of the reporter molecule. A near-infrared diode laser was used to excite efficiently the SERS signal while minimizing fluorescence interference. We show that, by using different labels with little spectral overlap, two different antigenic species can be detected simultaneously. The potential of this concept to function as a readout strategy for multiple analytes is briefly discussed.
引用
收藏
页码:4903 / 4908
页数:6
相关论文
共 42 条
  • [1] PROTEIN METAL INTERACTIONS IN PROTEIN-COLLOID CONJUGATES PROBED BY SURFACE-ENHANCED RAMAN-SPECTROSCOPY
    AHERN, AM
    GARRELL, RL
    [J]. LANGMUIR, 1991, 7 (02) : 254 - 261
  • [2] A RAPID AND SEMIQUANTITATIVE METHOD FOR THE DETECTION OF AUTOANTIBODIES BY MULTIPLE SPOT IMMUNOASSAY
    BASLUND, B
    WIESLANDER, J
    [J]. JOURNAL OF IMMUNOLOGICAL METHODS, 1994, 169 (02) : 183 - 193
  • [3] BAUDHUIN P, 1989, COLLOIDAL GOLD PRINC, V2, P1
  • [4] SIMULTANEOUS MEASUREMENT OF 5-CELL SURFACE-ANTIGENS BY 5-COLOR IMMUNOFLUORESCENCE
    BEAVIS, AJ
    PENNLINE, KJ
    [J]. CYTOMETRY, 1994, 15 (04): : 371 - 376
  • [5] BLAKE C, 1982, CLIN CHEM, V28, P1469
  • [6] Bohren C F., 1998, Absorption and Scattering of Light by Small Particles
  • [7] BROWN CR, 1985, CLIN CHEM, V31, P1500
  • [8] Quantized capacitance charging of monolayer-protected Au clusters
    Chen, SW
    Murray, RW
    Feldberg, SW
    [J]. JOURNAL OF PHYSICAL CHEMISTRY B, 1998, 102 (49): : 9898 - 9907
  • [9] Christopoulos Theodore K., 1996, P227, DOI 10.1016/B978-012214730-2/50011-X
  • [10] DONOHUE J, 1989, CLIN CHEM, V35, P1874