Development of a RT Real-Time PCR for the Detection and Quantification of Human Rhinoviruses

被引：30

作者：

Gambarino, Stefano ^{[1
]}

Costa, Cristina ^{[1
]}

Elia, Mariateresa ^{[1
]}

Sidoti, Francesca ^{[1
]}

Mantovani, Samantha ^{[1
]}

Gruosso, Valentina ^{[1
]}

Bergallo, Massimiliano ^{[1
]}

Cavallo, Rossana ^{[1
]}

机构：

[1] Univ Turin, Virol Unit, Dept Microbiol & Publ Hlth, I-10126 Turin, Italy

来源：

MOLECULAR BIOTECHNOLOGY | 2009年 / 42卷 / 03期

关键词：

Human Rhinoviruses; Real-time PCR (RT-PCR); Acute respiratory tract illness; Bronchoalveolar lavage; LOWER RESPIRATORY-TRACT; OBSTRUCTIVE PULMONARY-DISEASE; REVERSE TRANSCRIPTION-PCR; VIRUS; INFECTIONS; ASSAY; ENTEROVIRUS; TRANSPLANT; DIAGNOSIS; ASTHMA;

D O I：

10.1007/s12033-009-9164-x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

Human Rhinoviruses (HRV) are the most common viral agents, being responsible for upper as well as lower respiratory tract infections. Evidence demonstrating that HRV disease is not exclusively limited to the upper airways and may cause lower respiratory complications, together with the frequency of HRV infections and the increasing number of immunocompromised patients underline the need for including HRV in virological diagnostics of acute lower respiratory tract illness. This article describes the development and optimization of a reverse transcription (RT) real-time PCR assay for quantification of HRV RNA in clinical samples. Efficiency, sensitivity, specificity, inter- and intra-assay variability, and dynamic range have been determined. Subsequently, the assay has been validated on bronchoalveolar lavage (BAL) specimens obtained from immunocompetent and immunocompromised patients.

引用

页码：350 / 357

页数：8

共 22 条

[1]

Viral pathogens in acute exacerbations of chronic obstructive pulmonary disease [J].