Polysaccharide degradation by Fenton reaction- or peroxidase-generated hydroxyl radicals in isolated plant cell walls

被引：83

作者：

Schweikert, C ^{[1
]}

Liszkay, A ^{[1
]}

Schopfer, P ^{[1
]}

机构：

[1] Univ Freiburg, Inst Biol 2, D-79104 Freiburg, Germany

来源：

PHYTOCHEMISTRY | 2002年 / 61卷 / 01期

关键词：

cell wall; electron paramagnetic resonance (hydroxyl radical); Fenton reaction; horseradish peroxidase; hydroxyl radical; peroxidase; polysaccharide scission;

D O I：

10.1016/S0031-9422(02)00183-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The formation of hydroxyl radicals (OH.) by peroxidase was confirmed by EPR spectroscopy using ethanol/alpha-(4-pyridyl-1-oxide)-N-tert-butylnitrone as a spin-trapping system specific of OH.. The effect of OH., generated either non-enzymatically with the Fenton reaction (H2O2 + Fe2+) or with horseradish peroxidase in the presence of O-2 and NADH, on cell walls isolated from maize (Zea mays) coleoptiles or soybean (Glycine max) hypocotyls was investigated. OH. produced by these reactions attack polysaccharides in the wall, demonstrated by the release of a heterogeneous mixture of polymeric breakdown products into the incubation medium. The peroxidase-catalyzed degradation of cell-wall polysaccharides can be inhibited by KCN and superoxide radical (O-2(.-)) or OH. scavengers. These data support the hypothesis that OH., produced by cell-wall peroxidases in vivo, act as wall-loosening agents in plant extension growth. (C) 2002 Elsevier Science Ltd. All rights reserved.

引用

页码：31 / 35

页数：5

共 20 条

[1] Use of a new tetrazolium-based assay to study the production of superoxide radicals by tobacco cell cultures challenged with avirulent zoospores of Phytophthora parasitica var nicotianae [J].

Able, AJ ;

Guest, DI ;

Sutherland, MW .

PLANT PHYSIOLOGY, 1998, 117 (02) :491-499

[2] CHARACTERIZATION OF A SUPEROXIDE-DISMUTASE MIMIC PREPARED FROM DESFERRIOXAMINE AND MNO2 [J].

BEYER, WF ;

FRIDOVICH, I .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1989, 271 (01) :149-156

[3] STRUCTURAL MODELS OF PRIMARY-CELL WALLS IN FLOWERING PLANTS - CONSISTENCY OF MOLECULAR-STRUCTURE WITH THE PHYSICAL-PROPERTIES OF THE WALLS DURING GROWTH [J].

CARPITA, NC ;

GIBEAUT, DM .

PLANT JOURNAL, 1993, 3 (01) :1-30

[4] Hydroxyl-radical production in physiological reactions - A novel function of peroxidase [J].

Chen, SX ;

Schopfer, P .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1999, 260 (03) :726-735

[5] COLORIMETRIC METHOD FOR DETERMINATION OF SUGARS AND RELATED SUBSTANCES [J].

DUBOIS, M ;

GILLES, KA ;

HAMILTON, JK ;

REBERS, PA ;

SMITH, F .

ANALYTICAL CHEMISTRY, 1956, 28 (03) :350-356

[6] NADH-stimulated, cyanide-resistant superoxide production in maize coleoptiles analyzed with a tetrazolium-based assay [J].

Frahry, G ;

Schopfer, P .

PLANTA, 2001, 212 (02) :175-183

[7] Oxidative scission of plant cell wall polysaccharides by ascorbate-induced hydroxyl radicals [J].

Fry, SC .

BIOCHEMICAL JOURNAL, 1998, 332 :507-515

[8]

Halliwell B., 2001, FREE RADICALS BIOL M

[9] ACID-MEDIATED AND ENZYME-MEDIATED SOLUBILIZATION OF CELL-WALL BETA-1.3,BETA-1.4-D-GLUCAN IN MAIZE COLEOPTILES - IMPLICATIONS FOR AUXIN-MEDIATED GROWTH [J].

HOHL, M ;

HONG, YN ;

SCHOPFER, P .

PLANT PHYSIOLOGY, 1991, 95 (04) :1012-1018

[10] BETA-D-GLUCAN HYDROLASE ACTIVITY IN ZEA COLEOPTILE CELL-WALLS [J].

HUBER, DJ ;

NEVINS, DJ .

PLANT PHYSIOLOGY, 1980, 65 (05) :768-773

← 1 2 →