Gas channels for NH3:: Proteins from hyperthermophiles complement an Escherichia coli mutant

被引：34

作者：

Soupene, E

Chu, T

Corbin, RW

Hunt, DF

Kustu, S

机构：

[1] Univ Calif Berkeley, Dept Plant & Microbial Biol, Berkeley, CA 94720 USA

[2] Univ Virginia, Dept Chem, Charlottesville, VA 22901 USA

来源：

JOURNAL OF BACTERIOLOGY | 2002年 / 184卷 / 12期

关键词：

D O I：

10.1128/JB.184.12.3396-3400.2002

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Ammonium transport (Amt) proteins appear to be bidirectional channels for NH3. The amt genes of the hyperthermophiles Aquifex aeolicus and Methanococcus jannaschii complement enteric amtB mutants for growth at 25 nM NH3 at 37degreesC. To our knowledge. Amt proteins are the first hyperthermophilic membrane transport proteins shown to be active in a mesophilic bacterium. Despite low expression levels, His-tagged Aquifex Amt could be purified by heating and nickel chelate affinity chromatography. It could be studied genetically in Escherichia coli.

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页码：3396 / 3400

页数：5

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