In vitro ACE-inhibitory activity and in vivo antihypertensive effects of water-soluble extract by Parmigiano Reggiano and Grana Padano cheeses

被引：22

作者：

Bernabucci, Umberto ^{[1
]}

Catalani, Elisabetta ^{[1
]}

Basirico, Loredana ^{[1
]}

Morera, Patrizia ^{[1
]}

Nardone, Alessandro ^{[1
]}

机构：

[1] Univ Tuscia, Dept Agr Forests Nat & Energy, I-01100 Viterbo, Italy

来源：

INTERNATIONAL DAIRY JOURNAL | 2014年 / 37卷 / 01期

关键词：

CONVERTING-ENZYME; BLOOD-PRESSURE; PEPTIDES; VARIETIES;

D O I：

10.1016/j.idairyj.2014.02.009

中图分类号：

TS2 [食品工业];

学科分类号：

100403 [营养与食品卫生学];

摘要：

Angiotensin converting enzyme (ACE)-inhibitory activity and the anti-hypertensive effect of naturally formed bioactive peptides in a lyophilised water-soluble extract (WSE) from 32-month-aged Parmigiano Reggiano (PR) and Grana Padano (GP) cheeses were assessed. ACE-inhibitory activity was evaluated on undigested and in vitro digested WSE: consistent ACE-inhibitory activity in both hard cheeses was found. Twenty spontaneously hypertensive rats were used to test the hypotensive action of lyophilised WSEs from PR and GP cheeses; systolic (SBP) and diastolic (DBP) blood pressures were recorded by a non-invasive tail-cuff method. In vivo administration of GP and PR WSE did not induce a reduction of SBP and DBP after ten weeks of treatment. These results clearly indicate that in vitro ACE-inhibitory activity cannot be used as an exclusive criterion to evaluate potential anti-hypertensive substances. Therefore, in vivo testing of the anti-hypertensive effects of bioactive peptides is necessary to confirm beneficial effects. (C) 2014 Elsevier Ltd. All rights reserved.

引用

页码：16 / 19

页数：4

共 15 条

[1]

Tolerability of a Fully Maturated Cheese in Cow's Milk Allergic Children: Biochemical, Immunochemical, and Clinical Aspects [J].

Alessandri, Claudia ;

Sforza, Stefano ;

Palazzo, Paola ;

Lambertini, Francesca ;

Paolella, Sara ;

Zennaro, Danila ;

Rafaiani, Chiara ;

Ferrara, Rosetta ;

Bernardi, Maria Livia ;

Santoro, Mario ;

Zuzzi, Sara ;

Giangrieco, Ivana ;

Dossena, Arnaldo ;

Mari, Adriano .

PLOS ONE, 2012, 7 (07)

[2]

Butikofer U., 2007, INTERNATIONAL DAIRY, V17

[3]

Purification of angiotensin I-converting enzyme inhibitory peptides and antihypertensive effect of milk produced by protease-facilitated lactic fermentation [J].

Chen, Guan-Wen ;

Tsai, Jenn-Shou ;

Pan, Bonnie Sun .

INTERNATIONAL DAIRY JOURNAL, 2007, 17 (06) :641-647

[4]

FitzGerald RJ, 2000, BRIT J NUTR, V84, pS33

[5]

Angiotensin converting enzyme inhibitory peptides from a lactotripeptide-enriched milk beverage are absorbed intact into the circulation [J].

Foltz, Martin ;

Meynen, Evelyne E. ;

Bianco, Veronique ;

van Platerink, Chris ;

Koning, Thea M. M. G. ;

Kloek, Joris .

JOURNAL OF NUTRITION, 2007, 137 (04) :953-958

[6]

Antihypertensive Peptides from Milk Proteins [J].

Jakala, Pauliina ;

Vapaatalo, Heikki .

PHARMACEUTICALS, 2010, 3 (01) :251-272

[7]

Antihypertensive peptides from food proteins: a review [J].

Martinez-Maqueda, Daniel ;

Miralles, Beatriz ;

Recio, Isidra ;

Hernandez-Ledesma, Blanca .

FOOD & FUNCTION, 2012, 3 (04) :350-361

[8]

BLOOD-PRESSURE DEVELOPMENT OF THE SPONTANEOUSLY HYPERTENSIVE RAT AFTER CONCURRENT MANIPULATIONS OF DIETARY CA-2+ AND NA+ - RELATION TO INTESTINAL CA-2+ FLUXES [J].

MCCARRON, DA ;

LUCAS, PA ;

SHNEIDMAN, RJ ;

LACOUR, B ;

DRUEKE, T .

JOURNAL OF CLINICAL INVESTIGATION, 1985, 76 (03) :1147-1154

[9]

Identification of novel antihypertensive peptides in milk fermented with Enterococcus faecalis [J].

Quiros, Ana ;

Ramos, Mercedes ;

Muguerza, Begona ;

Delgado, Marco A. ;

Miguel, Marta ;

Aleixandre, Amaya ;

Recio, Isidra .

INTERNATIONAL DAIRY JOURNAL, 2007, 17 (01) :33-41

[10]

Antibacterial activities of peptides from the water-soluble extracts of Italian cheese varieties [J].

Rizzello, CG ;

Losito, I ;

Gobbetti, M ;

Carbonara, T ;

De Bari, MD ;

Zambonin, PG .

JOURNAL OF DAIRY SCIENCE, 2005, 88 (07) :2348-2360

← 1 2 →