Selective expression of the receptor tyrosine kinase, HTK, on human erythroid progenitor cells

被引:40
作者
Inada, T
Iwama, A
Sakano, S
Ohno, M
Sawada, K
Suda, T
机构
[1] KUMAMOTO UNIV, SCH MED, INST MOL EMBRYOL & GENET, DEPT CELL DIFFERENTIAT, KUMAMOTO 860, JAPAN
[2] KUMAMOTO UNIV, SCH MED, DEPT INTERNAL MED 2, KUMAMOTO 860, JAPAN
[3] ASAHI CHEM IND CO LTD, FUNDAMENTAL RES LAB LIFE SCI, SHIZUOKA, JAPAN
[4] HOKKAIDO UNIV, SCH MED, DEPT INTERNAL MED 2, SAPPORO, HOKKAIDO 060, JAPAN
关键词
D O I
10.1182/blood.V89.8.2757
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
HTK is a receptor tyrosine kinase of the Eph family. To characterize the involvement of HTK in hematopoiesis, we generated monoclonal antibodies against HTK and investigated its expression on human bone marrow cells. About 5% of the bone marrow cells were HTK+, which were also c-Kit(+), CD34(low), and glycophorin A(-/low). Assays of progenitors showed that HTK(+)c-Kit(+) cells consisted exclusively of erythroid progenitors, whereas HTK(-)c-Kit(+) cells contained progenitors of granulocytes and macrophages as well as those of erythroid cells. Most of the HTK+ erythroid progenitors were stem cell factor-dependent for proliferation, indicating that they represent mainly erythroid burst-forming units (BFU-E). During the erythroid differentiation of cultured peripheral CD34(+) cells, HTK expression was upregulated on immature erythroid cells that corresponded to BFU-E and erythroid colony-forming units and downregulated on erythroblasts with high levels of glycophorin expression. These findings suggest that HTK is selectively expressed on the restricted stage of erythroid progenitors, particularly BFU-E, and that HTK is the first marker antigen that allows the purification of erythroid progenitors. Furthermore, HTKL, the ligand for HTK, was expressed in the bone marrow stromal cells. Our findings provide a novel regulatory system of erythropoiesis mediated by the HTKL-HTK signaling pathway. (C) 1997 by The American Society of Hematology.
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页码:2757 / 2765
页数:9
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