Expression and localization of Menkes and Wilson copper transporting ATPases in human placenta

被引：38

作者：

Hardman, B

Manuelpillai, U

Wallace, EM

van de Waasenburg, S

Cater, M

Mercer, JFB

Ackland, ML

机构：

[1] Deakin Univ, Sch Biol & Chem Sci, Ctr Cellular & Mol Biol, Melbourne, Vic 3125, Australia

[2] Monash Univ, Monash Med Ctr, Dept Obstet & Gynaecol, Ctr Womens Hlth Res, Clayton, Vic 3168, Australia

来源：

PLACENTA | 2004年 / 25卷 / 06期

关键词：

D O I：

10.1016/j.placenta.2003.11.013

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Copper is an essential trace element necessary for normal growth and development. During pregnancy, copper is transported from. the maternal circulation to the fetus by mechanisms which have not been clearly elucidated. Two copper transporting ATPases, Menkes (ATP7A; MNK) and Wilson (ATP7B; WND) are known to be expressed in the placenta and are thought to have a role in copper transport to the fetus. In this study, the expression and localization of the MNK and WND proteins in the human placenta were investigated in detail using immunoperoxidase and double-label immunohistochemistry. MNK and WND are differentially localized within the placenta. MNK is present in the syncytiotrophoblast, the cytotrophoblast and the fetal vascular endothelial cells whereas WND is only in the syncytiotrophoblast. Placental levels of both proteins, measured by Western blot analysis, did not change across pregnancy. These data offer some insights into possible roles for MNK and WND within the placenta. (C) 2003 Elsevier Ltd. All rights reserved.

引用

页码：512 / 517

页数：6

共 37 条

[1] Expression of Menkes disease gene in mammary carcinoma cells [J].