Identification of carbonic anhydrase XII as the membrane isozyme expressed in the normal human endometrial epithelium

被引:73
作者
Karhumaa, P
Parkkila, S
Türeci, Ö
Waheed, A
Grubb, JH
Shah, G
Parkkila, AK
Kaunisto, K
Tapanainen, J
Sly, WS
Rajaniemi, H
机构
[1] Oulu Univ, Dept Anat & Cell Biol, FIN-90401 Oulu, Finland
[2] Oulu Univ, Dept Clin Chem, FIN-90401 Oulu, Finland
[3] Univ Saarland, Dept Internal Med, D-66421 Homburg, Germany
[4] St Louis Univ, Sch Med, Dept Biochem & Mol Biol, St Louis, MO 63104 USA
[5] Oulu Univ, Dept Obstet & Gynecol, FIN-90401 Oulu, Finland
关键词
bicarbonate; carbonic anhydrase; immunohistochemistry; pH; uterus;
D O I
10.1093/molehr/6.1.68
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
Although previous studies demonstrated carbonic anhydrase (CA) activity in the human endometrium, the CA isozyme(s) responsible for this activity has not been established. In this report, we provide the first evidence that the CA isozyme XII, a recently identified transmembrane isozyme that is expressed in normal kidney and greatly overexpressed in some renal cancers, is present in endometrium. We show by immunohistochemistry that CA XII is expressed in the basolateral plasma membrane of epithelial cells of normal human endometrium. Expression of CA XII in uterus was confirmed by Northern blotting. Detergent-solubilized CA XII was isolated from human endometrium by inhibitor affinity chromatography and characterized by isoelectric focusing and Western blot as a polypeptide with a pi of 6.3. The high expression of CA XII in the endometrial epithelium suggests that it may be functionally linked to the pH-dependent events in spermatozoa that precede fertilization. Its basolateral location and extracellular active site could also allow it to influence the morphological changes in endometrium that occur during the menstrual cycle.
引用
收藏
页码:68 / 74
页数:7
相关论文
共 43 条
[1]   THE STATUS AND THE STATE OF THE HUMAN EPIDIDYMIS [J].
BEDFORD, JM .
HUMAN REPRODUCTION, 1994, 9 (11) :2187-2199
[2]   Modelling human sperm-egg interactions in vitro: signal transduction pathways regulating the acrosome reaction [J].
Benoff, S .
MOLECULAR HUMAN REPRODUCTION, 1998, 4 (05) :453-471
[3]  
Boatman Dorothy E., 1997, Human Reproduction (Oxford), V12, P133
[4]   INHIBITION OF BOVINE SPERMATOZOA BY CAUDAL EPIDIDYMAL FLUID .1. STUDIES OF A SPERM MOTILITY QUIESCENCE FACTOR [J].
CARR, DW ;
ACOTT, TS .
BIOLOGY OF REPRODUCTION, 1984, 30 (04) :913-925
[5]   SINGLE-STEP METHOD OF RNA ISOLATION BY ACID GUANIDINIUM THIOCYANATE PHENOL CHLOROFORM EXTRACTION [J].
CHOMCZYNSKI, P ;
SACCHI, N .
ANALYTICAL BIOCHEMISTRY, 1987, 162 (01) :156-159
[6]  
FALK RJ, 1972, AM J OBSTET GYNECOL, V15, P1047
[7]   Cellular mechanisms of adrenaline-stimulated anion secretion by the mouse endometrial epithelium [J].
Fong, SK ;
Liu, CQ ;
Chan, HC .
BIOLOGY OF REPRODUCTION, 1998, 59 (06) :1342-1348
[8]   CELLULAR BIOLOGY OF CAPACITATION AND THE ACROSOME REACTION [J].
FRASER, LR .
HUMAN REPRODUCTION, 1995, 10 :22-30
[9]   CARBONIC-ANHYDRASE ACTIVITY IN MAMMALIAN OVARY, FALLOPIAN-TUBE, AND UTERUS - HISTOCHEMICAL AND BIOCHEMICAL STUDIES [J].
FRIEDLEY, NJ ;
ROSEN, S .
BIOLOGY OF REPRODUCTION, 1975, 12 (02) :293-304
[10]  
Guerin JF, 1997, HUM REPROD, V12, P1197