Identification and characterization of the functional amino acids at the active site of the large thioredoxin reductase from Plasmodium falciparum

The thioredoxin system, composed of the pyridine nucleotide disulfide oxidoreductase thioredoxin reductase, the small peptide thioredoxin, and NADPH as a reducing cofactor, is one of the major thiol-reducing systems of the cell, Recent studies revealed that Plasmodium falciparum and human thioredoxin reductase represent a novel class of enzymes, called large thioredoxin reductases, The large thioredoxin reductases are substantially different from the isofunctional prokaryotic Escherichia coli enzyme, The putative essential amino acids at the catalytic center of large thioredoxin reductase from P, falciparum were determined by using site-directed mutagenesis techniques, To analyze the putative active site cysteines (Cys(88) and Cys(93)) three mutant proteins were constructed substituting alanine or serine residues for cysteine residues, Further, to evaluate the function of His(509) as a putative proton donor/acceptor of large thioredoxin reductase this residue was replaced by either glutamine or alanine, All mutants were expressed in the E. coli system and characterized, Steady state kinetic analysis revealed that the replacement of Cys(88) by either alanine or serine and Cys(93) by alanine resulted in a total loss of enzymatic activity, These results clearly identify Cys(88) and Cys(93) as the active site thiols of large thioredoxin reductase. The replacement of His(509) by glutamine yielded in a 95% loss of thioredoxin reductase activity; replacement by alanine provoked a loss of 97% of enzymatic activity, These results identify His(509) as active site base, but imply that its function can be substituted, although inefficiently, by an alternative proton donor, similar to glutathione reductase, Spectral analysis of wild type P, falciparum thioredoxin reductase revealed a 550-nm absorption band upon reduction which resembles the EH2 form of glutathione reductase and lipoamide dehydrogenase. This spectral feature, recently also reported for the human placenta protein (Arscott, L, D,, Gromer, S,, Schirmer, R, H., Becker K., and Williams, C, H., Jr, (1997) PI oc. Natl, Acad. Sci, U, S, A, 94, 3621-3626), further illustrates the similarity between large thioredoxin reductases and glutathione reductases and stresses the profound differences to small E, coli thioredoxin reductase.