Whole Genome-expression Profiling Reveals a Role for Immune and Inflammatory Response in Abdominal Aortic Aneurysm Rupture

被引：70

作者：

Choke, E. ^{[1
]}

Cockerill, G. W. ^{[1
]}

Laing, K. ^{[2
]}

Dawson, J. ^{[1
]}

Willson, W. R. W. ^{[1
]}

Loftus, I. M. ^{[1
]}

Thompson, M. M. ^{[1
]}

机构：

[1] Univ London St Georges Hosp, St Georges Vasc Inst, London SW17 0RE, England

[2] Univ London, Dept Cellular & Mol Med, London SW17 0RE, England

来源：

EUROPEAN JOURNAL OF VASCULAR AND ENDOVASCULAR SURGERY | 2009年 / 37卷 / 03期

关键词：

Abdominal aortic aneurysm; Gene expression; Inflammation; Immune; Rupture; PROSTAGLANDIN E-2 SYNTHESIS; NECROSIS-FACTOR-ALPHA; GENE-EXPRESSION; INHIBITION; CYTOKINES; OVEREXPRESSION; CYCLOOXYGENASE; INTERLEUKIN-8; EXPANSION; DISEASE;

D O I：

10.1016/j.ejvs.2008.11.017

中图分类号：

R61 [外科手术学];

学科分类号：

摘要：

Objectives: This study used the whole transcriptome approach to investigate the role of genes involved in immune and inflammatory response at the site of aneurysm rupture. Materials and methods: Rupture site and paired anterior sac biopsies (internal control) of ruptured abdominal aortic aneurysms (AAAs) (n = 10) were analysed with Affymetrix Human Genome U133A plus 2.0 microarray. Twenty-one differentially expressed genes were selected for validation using quantitative reverse transcriptase polymerase chain reaction (QRT-PCR). Results: A total of 139 genes (123 upregulated, 16 downregulated) at the aneurysm rupture site were differentially expressed (>2.5-fold, P < 0.005). Immune and inflammatory responses (Gene Ontology Classification) were frequently associated with the differentially expressed genes. Genes with immune and inflammatory functions that were confirmed, by QRT-PCR, to be overexpressed at the aneurysm rupture site were interleukins-6 and -8 (IL-6 and -8), Selectin E (SELE), prostaglandin-endoperoxidase synthase 2 (COX2) and prokineticin 2 (PROK2). IL-6 (pro-immune) and IL-8 (pro-immune and pro-inflammatory) have previously been linked to aneurysm rupture; and SELE and COX2 (pro-inflammatory) have previous associations with aneurysm development but not rupture. Conclusions: The differential expression of genes involved in immune and inflammatory responses was confirmed at AAA rupture site. These genes may represent novel targets for treatment of aneurysms. (C) 2008 European Society for Vascular Surgery. Published by Elsevier Ltd. All rights reserved.

引用

页码：305 / 310

页数：6

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