Accumulation of 8-nitroguanine in human gastric epithelium induced by Helicobacter pylori infection

被引：74

作者：

Ma, N

Adachi, Y

Hiraku, Y

Horiki, N

Horiike, S

Imoto, I

Pinlaor, S

Murata, M

Semba, R

Kawanishi, S ^{[1
]}

机构：

[1] Mie Univ, Sch Med, Dept Environm & Mol Med, Tsu, Mie 5148507, Japan

[2] Mie Univ, Sch Med, Dept Internal Med, Tsu, Mie 5148507, Japan

[3] Mie Univ, Sch Med, Dept Endoscop Med, Tsu, Mie 5148507, Japan

[4] Mie Univ, Sch Med, Dept Anat, Tsu, Mie 5148507, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2004年 / 319卷 / 02期

关键词：

Helicobacter pylori; gastric cancer; DNA damage; 8-nitroguanine; 8-oxo-7,8-dihydro-2'-deoxyguanosine proliferating cell nuclear antigen; gastric gland epithelial cell; infection; inflammation; double immunofluorescence labeling;

D O I：

10.1016/j.bbrc.2004.04.193

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Helicobacter pylori infection causes chronic inflammation, which can lead to gastric carcinoma. A double immunofluorescence labeling study demonstrated that the level of 8-nitroguanine and 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG) apparent in gastric gland epithelium was significantly higher in gastritis patients with H. pylori infection than in those without infection. A significant accumulation of proliferating cell nuclear antigen, a prognostic factor for gastric cancer, was observed in gastric gland epithelial cells in patients with H. pylori infection as compared to those without infection, and its accumulation was closely correlated with the formation of 8-nitroguanine and 8-oxodG. These results suggest that nitrosative and oxidative DNA damage in gastric epithelial cells and their proliferation by H. pylori infection may lead to gastric carcinoma. 8-Nitroguanine could be not only a promising biomarker for inflammation but also a useful indicator of the risk of gastric cancer development in response to chronic H. pylori infection. (C) 2004 Elsevier Inc. All rights reserved.

引用

页码：506 / 510

页数：5

共 24 条

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