A genetic analysis of the role of calcineurin and calmodulin in Ca++-dependent improvement of NaCl tolerance of Saccharomyces cerevisiae

被引:20
作者
Danielsson, A [1 ]
Larsson, C [1 ]
Larsson, K [1 ]
Gustafsson, L [1 ]
Adler, L [1 ]
机构
[1] GOTHENBURG UNIV,DEPT GEN & MARINE MICROBIOL,LUNDBERG LAB,S-43190 GOTHENBURG,SWEDEN
关键词
calcineurin; calmodulin; Saccharomyces cerevisiae; salt tolerance;
D O I
10.1007/s002940050159
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
Mutants of Saccharomyces cerevisiae lacking activity of the Ca2+/calmodulin-dependent protein phosphatase calcineurin, show sensitivity to high concentrations of sodium that is partly reversed by the external supply of Ca2+ On long-time exposure to NaCl stress the mutants display an increased intracellular Na+/K+ ratio which is partially corrected by the addition of Ca2+, improving the sodium efflux of not only calcineurin-defective cells but also wild-type cells. We also demonstrate that the NaCl sensitivity of cmd mutants, expressing modified forms of calmodulin that do not bind Ca2+, is strongly reversed by the addition of Ca2+ This effect is highly dependent on calcineurin, since the NaCl tolerance of a cmdl-3 strain, carrying an additional mutation in calcineurin, is only slightly assisted by Ca2+ A striking characteristic of the loss of function of calcineurin is a several-fold increased content of intracellular Ca2+, localized mainly in subcellular compartment(s). If the compartmentalized Ca2+ pool is brought back to normal levels by an additional inactivating mutation of the vacuolar Ca2+ transporting ATPase, such double mutants do not significantly improve their tolerance to NaCl.
引用
收藏
页码:476 / 484
页数:9
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