Comparison of microwave-assisted extraction and conventional extraction techniques for the extraction of tanshinones from Salvia miltiorrhiza bunge

被引:128
作者
Pan, XJ [1 ]
Niu, GG [1 ]
Liu, HZ [1 ]
机构
[1] Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Young Scientist Lab Separat Sci & Engn, Beijing 100080, Peoples R China
基金
中国国家自然科学基金;
关键词
Salvia miltiorrhiza bunge; tanshinones; microwave-assisted; extraction method;
D O I
10.1016/S1369-703X(02)00039-6
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Extraction techniques using microwave-assisted extraction (MAE), extraction at room temperature (ERT), heat reflux extraction, ultrasonic extraction and Soxhlet extraction were evaluated for the extraction of tanshinones (Cryptotanshi none, Tanshinone I and Tanshinone IIA) from Salvia miltiorrhiza bunge. The extracts were analyzed by high performance liquid chromatography (HPLC) without any treatment. The results showed that the percentage extraction of Cryptotanshinone, Tanshinone I and Tanshinone IIA from S. miltiorrhiza bunge by MAE was equivalent with and in fact higher than that of conventional extraction methods. MAE only needs 2 min, whereas ERT, heat reflux extraction, ultrasonic extraction and Soxhlet extraction need 24 h, 45, 75 and 90 min, respectively. Due to the considerable saving of time and high extraction efficiency, MAE was more effective than the conventional methods. (C) 2002 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:71 / 77
页数:7
相关论文
共 20 条
[1]  
CHEN J, 1996, CHINESE TRADITIONAL, V18, P7
[2]   Extraction arad colorimetric determination of azadirachtin-related limonoids in neem seed kernel [J].
Dai, JM ;
Yaylayan, VA ;
Raghavan, GSV ;
Parè, JR .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 1999, 47 (09) :3738-3742
[3]   Extraction of Tanshinone IIA from Salvia miltiorrhiza bunge using supercritical fluid extraction and a new extraction technique, phytosol solvent extraction [J].
Dean, JR ;
Liu, B ;
Price, R .
JOURNAL OF CHROMATOGRAPHY A, 1998, 799 (1-2) :343-348
[4]   Microwave-assisted extraction of felodipine tablets [J].
Eskilsson, CS ;
Björklund, E ;
Mathiasson, L ;
Karlsson, L ;
Torstensson, A .
JOURNAL OF CHROMATOGRAPHY A, 1999, 840 (01) :59-70
[5]   MICROWAVE EXTRACTION - A NOVEL SAMPLE PREPARATION METHOD FOR CHROMATOGRAPHY [J].
GANZLER, K ;
SALGO, A ;
VALKO, K .
JOURNAL OF CHROMATOGRAPHY, 1986, 371 :299-306
[6]   Extraction of organic contaminants from marine sediments and tissues using microwave energy [J].
Jayaraman, S ;
Pruell, RJ ;
McKinney, R .
CHEMOSPHERE, 2001, 44 (02) :181-191
[7]   PHOTOOXIDATION OF TANSHINONE-II (6,7,8,9-TETRAHYDRO-1,6,6-TRIMETHYL-PHENANTHRO[1,2-B]FURAN-10,11-DIONE) [J].
KUSUMI, T ;
KISHI, T ;
KAKISAWA, H ;
KINOSHITA, T .
JOURNAL OF THE CHEMICAL SOCIETY-PERKIN TRANSACTIONS 1, 1976, (16) :1716-1718
[8]   ISOLATION AND BIOACTIVITY OF NEW TANSHINONES [J].
LEE, AR ;
WU, WL ;
CHANG, WL ;
LIN, HC ;
KING, ML .
JOURNAL OF NATURAL PRODUCTS, 1987, 50 (02) :157-160
[9]   Microwave-assisted extraction by fast sample preparation for the systematic analysis of additives in polyolefins by high-performance liquid chromatography [J].
Marcato, B ;
Vianello, M .
JOURNAL OF CHROMATOGRAPHY A, 2000, 869 (1-2) :285-300
[10]   Microwave-assisted extraction of taxanes from Taxus biomass [J].
Mattina, MJI ;
Berger, WAI ;
Denson, CL .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 1997, 45 (12) :4691-4696