Rapid Ertapenem Susceptibility Testing and Klebsiella pneumoniae Carbapenemase Phenotype Detection in Klebsiella pneumoniae Isolates by Use of Automated Microscopy of Immobilized Live Bacterial Cells

被引:23
作者
Burnham, Carey-Ann D. [1 ,2 ]
Frobel, Rachel A. [1 ]
Herrera, Monica L. [3 ]
Wickes, Brian L. [3 ]
机构
[1] Washington Univ, Sch Med, Dept Pathol & Immunol, St Louis, MO 63130 USA
[2] Washington Univ, Sch Med, Dept Pediat, St Louis, MO 63110 USA
[3] Univ Texas Hlth Sci Ctr San Antonio, Dept Microbiol & Immunol, San Antonio, TX 78229 USA
关键词
ENTEROBACTERIACEAE; EPIDEMIOLOGY; RESISTANCE; EMERGENCE; WATERS;
D O I
10.1128/JCM.03255-13
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
We evaluated detection of ertapenem (ETP) resistance and Klebsiella pneumoniae carbapenemase (KPC) in 47 Klebsiella pneumoniae isolates using a novel automated microscopy system. Automated microscopy correctly classified 22/23 isolates as ETP resistant and 24/24 as ETP susceptible and correctly classified 21/21 isolates as KPC positive and 26/26 as KPC negative.
引用
收藏
页码:982 / 986
页数:5
相关论文
共 18 条
  • [1] [Anonymous], 2013, WAYNE PENNSYLVANIA C
  • [2] Comparison of Meropenem MICs and Susceptibilities for Carbapenemase-Producing Klebsiella pneumoniae Isolates by Various Testing Methods
    Bulik, Catharine C.
    Fauntleroy, Kathy A.
    Jenkins, Stephen G.
    Abuali, Mayssa
    LaBombardi, Vincent J.
    Nicolau, David P.
    Kuti, Joseph L.
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 2010, 48 (07) : 2402 - 2406
  • [3] Genotype diversity of Escherichia coli isolates in natural waters determined by PFGE and ERIC-PCR
    Casarez, Elizabeth A.
    Pillai, Suresh D.
    Di Giouanni, George D.
    [J]. WATER RESEARCH, 2007, 41 (16) : 3643 - 3648
  • [4] National Surveillance Study on Carbapenem Non-Susceptible Klebsiella pneumoniae in Taiwan: The Emergence and Rapid Dissemination of KPC-2 Carbapenemase
    Chiu, Sheng-Kang
    Wu, Tsu-Lan
    Chuang, Yin-Ching
    Lin, Jung-Chung
    Fung, Chang-Phone
    Lu, Po-Liang
    Wang, Jann-Tay
    Wang, Lih-Shinn
    Siu, L. Kristopher
    Yeh, Kuo-Ming
    [J]. PLOS ONE, 2013, 8 (07):
  • [5] Detection of Klebsiella pneumoniae Carbapenemase (KPC) Production in Non-Klebsiella pneumoniae Enterobacteriaceae Isolates by Use of the Phoenix, Vitek 2, and Disk Diffusion Methods
    Doern, Christopher D.
    Dunne, W. Michael, Jr.
    Burnham, Carey-Ann D.
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 2011, 49 (03) : 1143 - 1147
  • [6] Emergence of carbapenem-resistant Enterobacteriaceae in a UK paediatric hospital
    Drew, R. J.
    Turton, J. F.
    Hill, R. L. R.
    Livermore, D. M.
    Woodford, N.
    Paulus, S.
    Cunliffe, N. A.
    [J]. JOURNAL OF HOSPITAL INFECTION, 2013, 84 (04) : 300 - 304
  • [7] Detection and treatment options for Klebsiella pneumoniae carbapenemases (KPCs): an emerging cause of multidrug-resistant infection
    Hirsch, Elizabeth B.
    Tam, Vincent H.
    [J]. JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 2010, 65 (06) : 1119 - 1125
  • [8] Crystal Structures of KPC-2 β-Lactamase in Complex with 3-Nitrophenyl Boronic Acid and the Penam Sulfone PSR-3-226
    Ke, Wei
    Bethel, Christopher R.
    Papp-Wallace, Krisztina M.
    Pagadala, Sundar Ram Reddy
    Nottingham, Micheal
    Fernandez, Daniel
    Buynak, John D.
    Bonomo, Robert A.
    van den Akker, Focco
    [J]. ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2012, 56 (05) : 2713 - 2718
  • [9] Carbapenem-Resistant Enterobacteriaceae : Laboratory Detection and Infection Control Practices
    Kruse, Eva-Brigitta
    Aurbach, Ute
    Wisplinghoff, Hilmar
    [J]. CURRENT INFECTIOUS DISEASE REPORTS, 2013, 15 (06) : 549 - 558
  • [10] ANALYSIS OF CLONAL RELATIONSHIPS AMONG ISOLATES OF SHIGELLA-SONNEI BY DIFFERENT MOLECULAR TYPING METHODS
    LIU, PYF
    LAU, YJ
    HU, BS
    SHYR, JM
    SHI, ZY
    TSAI, WS
    LIN, YH
    TSENG, CY
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 1995, 33 (07) : 1779 - 1783