Advances in loop-mediated isothermal amplification: integrated with several point-of-care diagnostic methods

被引:15
作者
Gong, Pin [1 ]
Zhang, Taotao [1 ]
Chen, Fuxin [2 ]
Wang, Lan [1 ]
Jin, Sai [1 ]
Bai, Xiaohuan [1 ]
机构
[1] Shaanxi Univ Sci & Technol, Coll Life Sci & Technol, Xian, Peoples R China
[2] Xian Univ Sci & Technol, Sch Chem & Chem Engn, Xian, Peoples R China
关键词
SPOT SYNDROME VIRUS; INFECTIOUS-DISEASES; VISUAL DETECTION; RAPID DETECTION; LAMP; SYSTEMS;
D O I
10.1039/c4ay00330f
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Recent outbreaks linked to pathogenic bacteria heighten the need to develop rapid, sensitive, portable and low-cost pathogen diagnostic systems. Loop-mediated isothermal amplification (LAMP) is a powerful and novel gene amplification method emerging as a simple rapid diagnostic tool for early detection and identification of microbial disease. Herein, we highlight the recent advances concerning LAMP which is integrated with several innovative detection technologies, thus, this article also reviews the extensive application of these basic techniques in food-borne bacterial detection.
引用
收藏
页码:7585 / 7589
页数:5
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