Rafts in adult peripheral nerve myelin contain major structural myelin proteins and myelin and lymphocyte protein (MAL) and CD59 as specific markers

被引:53
作者
Erne, B [1 ]
Sansano, S [1 ]
Frank, M [1 ]
Schaeren-Wiemers, N [1 ]
机构
[1] Univ Basel Hosp, Pharmactr, Dept Res, CH-4031 Basel, Switzerland
关键词
glycolipid-enriched microdomains; myelin maintenance; polarized transport; proteolipids; sorting;
D O I
10.1046/j.1471-4159.2002.00987.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The myelin and lymphocyte protein (MAL) proteolipid is localized in central and peripheral compact myelin membranes, as well as in apical membranes of particular polarized cells. In this study, we addressed the question whether MAL and other peripheral myelin proteins are sorted and targeted to myelin membranes using mechanisms similar to those observed in polarized epithelial cells. To investigate the presence of raft-mediated sorting pathways in Schwann cells, we have isolated and analysed their composition in myelin membranes. Here, we show that rafts are present in adult human and rat peripheral compact myelin membranes and contain MAL, the GPI-anchored protein CD59, and substantial amounts of the PMP22 and P0. Colocalization studies show that CD59, and MAL have an almost identical expression pattern within compact myelin. Moreover, immuno-electron microscopy revealed that MAL, besides its localization in compact myelin, is also localized to Schmidt-Lanterman incisures. Taken together, our results demonstrate the presence of detergent-insoluble glycolipid-enriched complexes (DIGs) in different compartments of myelin membranes and indicate an important role for DIG-mediated transport mechanisms in the maintenance of the adult myelin sheath.
引用
收藏
页码:550 / 562
页数:13
相关论文
共 51 条
[1]   DCNA CLONING AND SEQUENCE OF MAL, A HYDROPHOBIC PROTEIN ASSOCIATED WITH HUMAN T-CELL DIFFERENTIATION [J].
ALONSO, MA ;
WEISSMAN, SM .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (07) :1997-2001
[2]   PRODUCTION AND CHARACTERIZATION OF MONOCLONAL-ANTIBODIES TO THE EXTRACELLULAR DOMAIN OF PO [J].
ARCHELOS, JJ ;
ROGGENBUCK, K ;
SCHNEIDERSCHAULIES, J ;
LININGTON, C ;
TOYKA, KV ;
HARTUNG, HP .
JOURNAL OF NEUROSCIENCE RESEARCH, 1993, 35 (01) :46-53
[3]   On the molecular architecture of myelinated fibers [J].
Arroyo, EJ ;
Scherer, SS .
HISTOCHEMISTRY AND CELL BIOLOGY, 2000, 113 (01) :1-18
[4]   THE EFFECT OF THE SHIVERER MUTATION ON MYELIN BASIC-PROTEIN EXPRESSION IN HOMOZYGOUS AND HETEROZYGOUS MOUSE-BRAIN [J].
BARBARESE, E ;
NIELSON, ML ;
CARSON, JH .
JOURNAL OF NEUROCHEMISTRY, 1983, 40 (06) :1680-1686
[5]   Functional breakdown of the lipid bilayer of the myelin membrane in central and peripheral nervous system by disrupted galactocerebroside synthesis [J].
Bosio, A ;
Binczek, E ;
Stoffel, W .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1996, 93 (23) :13280-13285
[6]   SORTING OF GPI-ANCHORED PROTEINS TO GLYCOLIPID-ENRICHED MEMBRANE SUBDOMAINS DURING TRANSPORT TO THE APICAL CELL-SURFACE [J].
BROWN, DA ;
ROSE, JK .
CELL, 1992, 68 (03) :533-544
[7]   THE EPITOPE(S) RECOGNIZED BY HNK-1 ANTIBODY AND IGM PARAPROTEIN IN NEUROPATHY IS PRESENT ON SEVERAL N-LINKED OLIGOSACCHARIDE STRUCTURES ON HUMAN P0 AND MYELIN-ASSOCIATED GLYCOPROTEIN [J].
BURGER, D ;
SIMON, M ;
PERRUISSEAU, G ;
STECK, AJ .
JOURNAL OF NEUROCHEMISTRY, 1990, 54 (05) :1569-1575
[8]   2 MEMBRANE-PROTEIN FRACTIONS FROM RAT CENTRAL MYELIN WITH INHIBITORY PROPERTIES FOR NEURITE GROWTH AND FIBROBLAST SPREADING [J].
CARONI, P ;
SCHWAB, ME .
JOURNAL OF CELL BIOLOGY, 1988, 106 (04) :1281-1288
[9]   VIP17/MAL, a lipid raft-associated protein, is involved in apical transport in MDCK cells [J].
Cheong, KH ;
Zacchetti, D ;
Schneeberger, EE ;
Simons, K .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1999, 96 (11) :6241-6248
[10]   Myelination in the absence of galactocerebroside and sulfatide: Normal structure with abnormal function and regional instability [J].
Coetzee, T ;
Fujita, N ;
Dupree, J ;
Shi, R ;
Blight, A ;
Suzuki, K ;
Suzuki, K ;
Popko, B .
CELL, 1996, 86 (02) :209-219