Disruption of TrkB-mediated signaling induces disassembly of postsynaptic receptor clusters at neuromuscular junctions

被引：199

作者：

Gonzalez, M ^{[1
]}

Ruggiero, FP ^{[1
]}

Chang, Q ^{[1
]}

Shi, YJ ^{[1
]}

Rich, MM ^{[1
]}

Kraner, S ^{[1
]}

Balice-Gordon, RJ ^{[1
]}

机构：

[1] Univ Penn, Sch Med, Dept Neurosci, Philadelphia, PA 19104 USA

来源：

NEURON | 1999年 / 24卷 / 03期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1016/S0896-6273(00)81113-7

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Neurotrophins and tyrosine receptor kinase (Trk) receptors are expressed in skeletal muscle, but it is unclear what functional role Trk-mediated signaling plays during postnatal life. Full-length TrkB (trkB.FL) as well as truncated TrkB (trkB.t1) were found to be localized primarily to the postsynaptic acetylcholine receptor- (AChR-) rich membrane at neuromuscular junctions. In vivo, dominant-negative manipulation of TrkB signaling using adenovirus to overexpress trkB.t1 in mouse sternomastoid muscle fibers resulted in the disassembly of postsynaptic AChR clusters at neuromuscular junctions, similar to that observed in mutant trkB(+/-) mice. When TrkB-mediated signaling was disrupted in cultured myotubes in the absence of motor nerve terminals and Schwann cells, agrin-induced AChR clusters were also disassembled. These results demonstrate a novel role for neurotrophin signaling through TrkB receptors on muscle fibers in the ongoing maintenance of postsynaptic AChR regions.

引用

页码：567 / 583

页数：17

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