Development of a genetic selection for catalytic antibodies

被引：2

作者：

Gildersleeve, J ^{[1
]}

Janes, J ^{[1
]}

Ulrich, H ^{[1
]}

Yang, P ^{[1
]}

Barbas, C ^{[1
]}

Schultz, PG ^{[1
]}

机构：

[1] Scripps Res Inst, Dept Chem, La Jolla, CA 92037 USA

来源：

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS | 2002年 / 12卷 / 12期

关键词：

D O I：

10.1016/S0960-894X(02)00198-1

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

The design and evaluation of a new genetic selection system for evolving catalytic antibodies with aldolase activity are described. Through a series of model selections, we have identified selection conditions where expression of a catalytically active antibody confers a growth advantage to Escherichia coli. In addition, we provide evidence that the growth advantage is a direct result of catalytic activity. (C) 2002 Published by Elsevier Science Ltd.

引用

页码：1691 / 1694

页数：4

共 8 条

[1]

KUSR P, 1996, PURE APPL CHEM, V68, P2017

[2] A GENETIC APPROACH TO THE GENERATION OF ANTIBODIES WITH ENHANCED CATALYTIC ACTIVITIES [J].

LESLEY, SA ;

PATTEN, PA ;

SCHULTZ, PG .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (04) :1160-1165

[3] Multiple event activation of a generic prodrug trigger by antibody catalysis [J].

Shabat, D ;

Rader, C ;

List, B ;

Lerner, RA ;

Barbas, CF .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1999, 96 (12) :6925-6930

[4] SELECTION OF CATALYTIC ANTIBODIES FOR A BIOSYNTHETIC REACTION FROM A COMBINATORIAL CDNA LIBRARY BY COMPLEMENTATION OF AN AUXOTROPHIC ESCHERICHIA COLI - ANTIBODIES FOR OROTATE DECARBOXYLATION [J].

SMILEY, JA ;

BENKOVIC, SJ .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (18) :8319-8323

[5] INVIVO CATALYSIS OF A METABOLICALLY ESSENTIAL REACTION BY AN ANTIBODY [J].

TANG, Y ;

HICKS, JB ;

HILVERT, D .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (19) :8784-8786

[6] EXPRESSION STUDIES OF CATALYTIC ANTIBODIES [J].

ULRICH, HD ;

PATTEN, PA ;

YANG, PL ;

ROMESBERG, FE ;

SCHULTZ, PG .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1995, 92 (25) :11907-11911

[7] EFFICIENT ALDOLASE CATALYTIC ANTIBODIES THAT USE THE ENAMINE MECHANISM OF NATURAL ENZYMES [J].

WAGNER, J ;

LERNER, RA ;

BARBAS, CF .

SCIENCE, 1995, 270 (5243) :1797-1800

[8] MOLECULAR STUDIES ON ENZYMES IN CHORISMATE METABOLISM AND THE ENTEROBACTIN BIOSYNTHETIC-PATHWAY [J].

WALSH, CT ;

LIU, J ;

RUSNAK, F ;

SAKAITANI, M .

CHEMICAL REVIEWS, 1990, 90 (07) :1105-1129

← 1 →