Evaluation of fluconazole resistance mechanisms in Candida albicans clinical isolates from HIV-infected patients in Brazil

被引：101

作者：

Goldman, GH ^{[1
]}

Ferreira, MED

Marques, ED

Savoldi, M

Perlin, D

Park, S

Martinez, PCG

Goldman, MHS

Colombo, AL

机构：

[1] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, BR-05508 Sao Paulo, Brazil

[2] Publ Hlth Res Inst, New York, NY USA

[3] Univ Fed Sao Paulo, Div Infect Dis, Sao Paulo, Brazil

[4] Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Pret, BR-05508 Sao Paulo, Brazil

来源：

DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE | 2004年 / 50卷 / 01期

基金：

巴西圣保罗研究基金会;

关键词：

D O I：

10.1016/j.diagmicrobio.2004.04.009

中图分类号：

R51 [传染病];

学科分类号：

100401 [流行病与卫生统计学];

摘要：

In this study, we describe resistance mechanisms in fluconazole-resistant isolates of C albicans isolated from AIDS patients from nine Brazilian hospitals. These mechanisms include the presence of point mutations in the ERG11 gene and overexpression of ERG11, and several genes encoding efflux pumps, as measured by quantitative real-time reverse transcriptase polymerase chain reaction. Several fluconazole-resistant strains had multiple mechanisms of resistance. Four mutations previously described, Y132F, K143R, E266D, and V4371, were identified among the strains, whereas some isolates contained more than one mutation. Fourteen novel mutations were identified. Interestingly, all Brazilian fluconazole-resistant isolates showed homozygosity at mating-type loci (MTL) associated with fluconazole resistance. This is the first comprehensive assessment at molecular level of mechanisms of fluconazole resistance in C albicans isolates from South America. (C) 2004 Elsevier Inc. All rights reserved.

引用

页码：25 / 32

页数：8

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