High variability between results of different in-house tests for cytomegalovirus (CMV) monitoring and a standardized quantitative plasma CMV PCR assay

被引:31
作者
von Müller, L
Hampl, W
Hinz, J
Meisel, H
Reip, A
Engelmann, E
Heilbronn, R
Gärtner, B
Krämer, O
Einsele, H
Hebart, H
Ljubicic, T
Löffler, J
Mertens, T
机构
[1] Univ Ulm, Abt Virol, Ulm, Germany
[2] Charite Berlin, Inst Virol, Berlin, Germany
[3] Free Univ Berlin, Inst Virol, D-1000 Berlin, Germany
[4] Univ Homburg, Abt Virol, D-6650 Homburg, Germany
[5] Univ Tubingen, Med Klin & Poliklin, Abt 2, D-7400 Tubingen, Germany
关键词
D O I
10.1128/JCM.40.6.2285-2287.2002
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
A total of 2,718 blood samples were analyzed in five virological laboratories for the presence of cytomegalovirus (CNW) by in-house tests and one standardized plasma PCR assay. Results from in-house tests showed remarkable variability. Detection of CNW pp65 antigen or DNA from cells was more sensitive than that by plasma CMV PCR assay.
引用
收藏
页码:2285 / 2287
页数:3
相关论文
共 11 条
  • [1] Plasma polymerase chain reaction for cytomegalovirus DNA after allogeneic marrow transplantation - Comparison with polymerase chain reaction using peripheral blood leukocytes, pp65 antigenemia, and viral culture
    Boeckh, M
    GallezHawkins, GM
    Myerson, D
    Zaia, JA
    Bowden, RA
    [J]. TRANSPLANTATION, 1997, 64 (01) : 108 - 113
  • [2] Quantitation of cytomegalovirus: Methodologic aspects and clinical applications
    Boeckh, M
    Boivin, G
    [J]. CLINICAL MICROBIOLOGY REVIEWS, 1998, 11 (03) : 533 - +
  • [3] Britt W., 1996, Fields Virology, P2493
  • [4] Comparison of quantitative and qualitative PCR assays for cytomegalovirus DNA in plasma
    Caliendo, AM
    Schuurman, R
    Yen-Lieberman, B
    Spector, SA
    Andersen, J
    Manjiry, R
    Crumpacker, C
    Lurain, NS
    Erice, A
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 2001, 39 (04) : 1334 - 1338
  • [5] EINSELE H, 1995, BLOOD, V86, P2815
  • [6] Application of viral-load kinetics to identify patients who develop cytomegalovirus disease after transplantation
    Emery, VC
    Sabin, CA
    Cope, AV
    Gor, D
    Hassan-Walker, AF
    Griffiths, PD
    [J]. LANCET, 2000, 355 (9220) : 2032 - 2036
  • [7] THE LOWER MATRIX PROTEIN-PP65 IS THE PRINCIPAL VIRAL-ANTIGEN PRESENT IN PERIPHERAL-BLOOD LEUKOCYTES DURING AN ACTIVE CYTOMEGALOVIRUS-INFECTION
    GREFTE, JMM
    VANDERGUN, BTF
    SCHMOLKE, S
    VANDERGIESSEN, M
    VANSON, WJ
    PLACHTER, B
    JAHN, G
    THE, TH
    [J]. JOURNAL OF GENERAL VIROLOGY, 1992, 73 : 2923 - 2932
  • [8] HERTENSTEIN B, 1995, BONE MARROW TRANSPL, V15, P387
  • [9] Prösch S, 1998, J CLIN MICROBIOL, V36, P3636
  • [10] The humoral immune response against human cytomegalovirus is characterized by a delayed synthesis of glycoprotein-specific antibodies
    Schoppel, K
    Kropff, B
    Schmidt, C
    Vornhagen, R
    Mach, M
    [J]. JOURNAL OF INFECTIOUS DISEASES, 1997, 175 (03) : 533 - 544