Isolation and metabolic characterization of a Pseudomonas stutzeri mutant able to grow on the three isomers of xylene

被引:20
作者
DiLecce, C [1 ]
Accarino, M [1 ]
Bolognese, F [1 ]
Galli, E [1 ]
Barbieri, P [1 ]
机构
[1] UNIV MILAN,DIPARTIMENTO GENET & BIOL MICROORGANISMI,I-20133 MILAN,ITALY
关键词
D O I
10.1128/AEM.63.8.3279-3281.1997
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
From an o-xylene-degrading Pseudomonas stutzeri strain (OX1), we previously isolated mutant M1, which had acquired the ability to grow on m-xylene and p-xylene but lost the ability to utilize the ortho isomer. From M1 cultures we have now isolated a revertant strain (R1) which grows on o-xylene and retains the ability to grow with the meta and para isomers regardless of the selective pressure applied. In P. stutzeri R1, o-xylene is degraded through two successive monooxygenations of the aromatic ring, while m-xylene and p-xylene catabolism proceeds through the progressive oxidation of a methyl substituent, although unquantifiable amounts of these two substrates are transformed into the corresponding dimethylphenols, which are not utilized for farther growth. The two catabolic pathways are inducible by all three xylene isomers.
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收藏
页码:3279 / 3281
页数:3
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