Single Lgr5 stem cells build crypt-villus structures in vitro without a mesenchymal niche

被引：5797

作者：

Sato, Toshiro ^{[1
,2
]}

Vries, Robert G. ^{[1
,2
]}

Snippert, Hugo J. ^{[1
,2
]}

van de Wetering, Marc ^{[1
,2
]}

Barker, Nick ^{[1
,2
]}

Stange, Daniel E. ^{[1
,2
]}

van Es, Johan H. ^{[1
,2
]}

Abo, Arie ^{[3
]}

Kujala, Pekka ^{[4
]}

Peters, Peter J. ^{[4
]}

Clevers, Hans ^{[1
,2
]}

机构：

[1] Hubrecht Inst, NL-3584 CT Utrecht, Netherlands

[2] Univ Med Ctr Utrecht, NL-3584 CT Utrecht, Netherlands

[3] Nuvelo Inc, San Carlos, CA 94070 USA

[4] Antoni Van Leeuwenhoek Hosp, Netherlands Canc Inst, NL-1066 CX Amsterdam, Netherlands

来源：

NATURE | 2009年 / 459卷 / 7244期

关键词：

SMALL-INTESTINE; EPITHELIAL-CELLS; EXPRESSION; DIFFERENTIATION; PROLIFERATION; INHIBITION; CULTURES; GROWTH; COLON; MICE;

D O I：

10.1038/nature07935

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

070301 [无机化学]; 070403 [天体物理学]; 070507 [自然资源与国土空间规划学]; 090105 [作物生产系统与生态工程];

摘要：

The intestinal epithelium is the most rapidly self-renewing tissue in adult mammals. We have recently demonstrated the presence of about six cycling Lgr(5+) stem cells at the bottoms of small-intestinal crypts(1). Here we describe the establishment of long-term culture conditions under which single crypts undergo multiple crypt fission events, while simultanously generating villus-like epithelial domains in which all differentiated cell types are present. Single sorted Lgr(5+) stem cells can also initiate these crypt-villus organoids. Tracing experiments indicate that the Lgr(5+) stem-cell hierarchy is maintained in organoids. We conclude that intestinal crypt-villus units are self-organizing structures, which can be built from a single stem cell in the absence of a non-epithelial cellular niche.

引用

页码：262 / U147

页数：5

共 30 条

[1]

The intestinal stem cell [J].