In vitro culture selection increases glyphosate tolerance in barley

被引：10

作者：

Escorial, MC ^{[1
]}

Sixto, H ^{[1
]}

GarciaBaudin, JM ^{[1
]}

Chueca, MC ^{[1
]}

机构：

[1] INIA,CIT,AREA PROTECC VEGETAL,MADRID 28040,SPAIN

来源：

PLANT CELL TISSUE AND ORGAN CULTURE | 1996年 / 46卷 / 03期

关键词：

cereal; herbicide-tolerance; Hordeum vulgare; somaclonal variation;

D O I：

10.1007/BF02307093

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

In vitro culture of barley calluses has been used to produce plants with increased glyphosate tolerance. Calluses from immature embryos of barley Hordeum vulgare L. 'Jeff') were cultured on Murashige and Skoog medium with 10(-6), 10(-5), 10(-4), 5x10(-4), 10(-3), or 10(-2)M glyphosate for one, four or thirty months. Plants were regenerated from calluses maintained in glyphosate medium at 10(-6), 10(-5) or 10(-4)M for four months, at 10(-5) or 5x10(-4)M for one month and at 10(-5)M for thirty months. The progeny of each regenerated plant was analyzed for response to glyphosate. Some progenies showed increased tolerance to glyphosate.

引用

页码：179 / 186

页数：8

共 30 条

[1] BIOCHEMICAL BASIS FOR GLYCOPHOSPHATE-TOLERANCE IN A BACTERIUM AND A PLANT-TISSUE CULTURE [J].