Multiresistance genes of Rhizobium etli CFN42

被引：83

作者：

González-Pasayo, R

Martínez-Romero, E

机构：

[1] Univ Nacl Autonoma Mexico, Ctr Invest Sobre Fijac Nitrogeno, Cuernavaca 62191, Morelos, Mexico

[2] Univ Nacl Autonoma Mexico, Fac Ciencias, Cuernavaca 62191, Morelos, Mexico

来源：

MOLECULAR PLANT-MICROBE INTERACTIONS | 2000年 / 13卷 / 05期

关键词：

D O I：

10.1094/MPMI.2000.13.5.572

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Multidrug efflux pumps of bacteria are involved in the resistance to various antibiotics and toxic compounds. In Rhizobium etli, a mutualistic symbiont of Phaseolus vulgaris (bean), genes resembling multidrug efflux pump genes were identified and designated rmrA and rmrB. rmrA was obtained after the screening of transposon-generated fusions that are inducible by bean-root released flavonoids, The predicted gene products of rmrAB shared significant homology to membrane fusion and major facilitator proteins, respectively. Mutants of rmrA formed on average 40% less nodules in bean, while mutants of rmrA and rmrB had enhanced sensitivity to phytoalexins, flavonoids, and salicylic acid, compared with the wild-type strain. Multidrug resistance genes emrAB from Escherichia coli complemented an rmrA mutant from R, etli for resistance to high concentrations of naringenin.

引用

页码：572 / 577

页数：6

共 49 条

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