High concentrations of ascorbic acid induces apoptosis of human gastric cancer cell by p38-MAP kinase-dependent up-regulation of transferrin receptor

被引：34

作者：

Ha, Yu Mi

Park, Min Kyu

Kim, Hye Jung

Seo, Han Geuk

Lee, Jae Heun

Chang, Ki Churl ^{[1
]}

机构：

[1] Gyeongsang Natl Univ, Sch Med, Dept Pharmacol, Jinju 660751, South Korea

来源：

CANCER LETTERS | 2009年 / 277卷 / 01期

关键词：

Apoptosis; Ascorbic acid; Transferrin receptor; p38-MAPK; Hydrogen peroxide; HYDROGEN-PEROXIDE; OXIDATIVE STRESS; VITAMIN-C; IRON UPTAKE; ENDOTHELIAL-CELLS; RADICAL INTENSITY; DOWN-REGULATION; CULTURE-MEDIUM; IN-VITRO; MAINTENANCE;

D O I：

10.1016/j.canlet.2008.11.020

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 [肿瘤学];

摘要：

We investigated the molecular mechanism by which ascorbic acid (AA) induces apoptosis in human gastric cancer cells, AGS cells. High concentration (more than 5 mM) of AA increased cellular iron uptake by increasing transferrin receptor (TfR) expression and induced AGS cell apoptosis which was inhibited by catalase. Interestingly, p38 mitogen-activated protein kinase (MAPK) inhibitor inhibited the upregulation of TfR and increased cell survival by AA. TfR-siRNA-transfected cells reduced apoptosis by AA. H2O2 increased TfR expression in AGS cells. Taken together, we concluded that high concentration of AA, through H2O2, induces apoptosis of AGS cells by p38-MAPK-dependent upregulation of TfR. (C) 2008 Elsevier Ireland Ltd. All rights reserved.

引用

页码：48 / 54

页数：7

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