Genomic fingerprinting of the 16S-23S gene spacer region suggests that novel Acinetobacter isolates are present in activated sludge

被引：4

作者：

Carr, E ^{[1
]}

Seviour, RJ

Gürtler, V

机构：

[1] La Trobe Univ, Biotechnol Res Ctr, Bendigo, Vic, Australia

[2] Austin & Repatriat Med Ctr, Dept Microbiol, Heidelberg, Vic, Australia

来源：

WATER SCIENCE AND TECHNOLOGY | 2002年 / 46卷 / 1-2期

关键词：

Acinetobacter; activated sludge; phosphorus removal; 16S-23S rDNA spacer region;

D O I：

10.2166/wst.2002.0515

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

The taxonomic status of the genus Acinetobacter is currently confused and the role of these organisms in activated sludge is poorly understood. Currently unidentified isolates of Acinetobacter from activated sludge were fingerprinted by making use of polymorphisms in their 16S-23S rDNA spacer region The PCR amplified 16S-23S rDNA spacer region was digested with five different restriction enzymes to further differentiate between the isolates. The resulting band patterns were very diverse and the data suggests that the activated sludge isolates are different to the known genomic species of Acinetobacter which are predominantly clinical isolates. The results of this study imply the existence of yet unrecognised species of Acinetobacter in activated sludge.

引用

页码：449 / 452

页数：4

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