BK virus regulatory region rearrangements in brain and cerebrospinal fluid from a leukemia patient with tubulointerstitial nephritis and meningoencephalitis

被引:49
作者
Stoner, GL
Alappan, R
Jobes, DV
Ryschkewitsch, CF
Landry, ML
机构
[1] NINCDS, Neurotoxicol Sect, NIH, Bethesda, MD 20892 USA
[2] Yale Univ, Sch Med, Dept Lab Med, New Haven, CT USA
关键词
BK virus (BKV); nephritis; leukemia; meningoencephalitis; virology; cerebrospinal fluid (CSF); urine;
D O I
10.1053/ajkd.2002.32795
中图分类号
R5 [内科学]; R69 [泌尿科学(泌尿生殖系疾病)];
学科分类号
1002 ; 100201 ;
摘要
BK virus (BKV) was recovered by polymerase chain reaction (PCR) from brain, kidney, lung, urine, and cerebrospinal fluid (CSF) of a fatal case of BKV tubulointerstitial nephritis with dissemination to lung and brain. Viral regulatory regions in PCR-amplified urine and the lung samples were identical to the archetypal structure, WWT, In the brain and CSF, a rearranged sequence predominated, however. A 94-bp deletion preceded a 71-bp tandem duplication because the same 94-bp segment was deleted from both copies. PCR-amplifled regulatory region products were cloned and sequenced to define further the extent of the rearranged structures. Two kidney clones were archetypal, whereas two others were rearranged differently from the brain and from each other. In contrast to the brain clones, the kidney rearrangements seemed to involve deletion after duplication. Three of four brain clones sequenced were identical to the rearrangement found to dominate in the PCR product. A fourth clone showed two short deletions without any duplication. The four CSF clones all showed rearrangements Identical to that which was amplified by PCR from CSF and brain. This represents the first molecular analysis of a BKV strain obtained from a central nervous system infection, and it reveals regulatory region rearrangements reminiscent of those described in JC virus from brains with progressive multifocal leukoencephalopathy. We suggest that the presence in the CSF of BKV with a dominant rearranged regulatory region may be useful in the diagnosis of BKV meningoencephalitis secondary to BKV nephritis. (C) 2002 by the National Kidney Foundation, Inc.
引用
收藏
页码:1102 / 1112
页数:11
相关论文
共 47 条
[1]   JC virus regulatory region rearrangements and genotypes in progressive multifocal leukoencephalopathy: Two independent aspects of virus variation [J].
Agostini, HT ;
Ryschkewitsch, CF ;
Singer, EJ ;
Stoner, GL .
JOURNAL OF GENERAL VIROLOGY, 1997, 78 :659-664
[2]   BK VIRUS AND A NEW-TYPE OF JC VIRUS EXCRETED BY HIV-1 POSITIVE PATIENTS IN RURAL TANZANIA [J].
AGOSTINI, HT ;
BRUBAKER, GR ;
SHAO, J ;
LEVIN, A ;
RYSHKEWITSCH, CF ;
BLATTNER, WA ;
STONER, GL .
ARCHIVES OF VIROLOGY, 1995, 140 (11) :1919-1934
[3]   Genotype profile of human polyomavirus JC excreted in urine of immunocompetent individuals [J].
Agostini, HT ;
Ryschkewitsch, CF ;
Stoner, GL .
JOURNAL OF CLINICAL MICROBIOLOGY, 1996, 34 (01) :159-164
[4]  
Agostini HT, 2001, HUMAN POLYOMAVIRUSES, P491
[5]  
[Anonymous], VIRUS GENES
[6]  
ARTHUR RR, 1989, PROG MED VIROL, V36, P42
[7]   BK-VIRUS AND JC-VIRUS INFECTIONS IN RECIPIENTS OF BONE-MARROW TRANSPLANTS [J].
ARTHUR, RR ;
SHAH, KV ;
CHARACHE, P ;
SARAL, R .
JOURNAL OF INFECTIOUS DISEASES, 1988, 158 (03) :563-569
[8]   ASSOCIATION OF BK VIRURIA WITH HEMORRHAGIC CYSTITIS IN RECIPIENTS OF BONE-MARROW TRANSPLANTS [J].
ARTHUR, RR ;
SHAH, KV ;
BAUST, SJ ;
SANTOS, GW ;
SARAL, R .
NEW ENGLAND JOURNAL OF MEDICINE, 1986, 315 (04) :230-234
[9]   HUMAN POLYOMAVIRUS JC PROMOTER ENHANCER REARRANGEMENT PATTERNS FROM PROGRESSIVE MULTIFOCAL LEUKOENCEPHALOPATHY BRAIN ARE UNIQUE DERIVATIVES OF A SINGLE ARCHETYPAL STRUCTURE [J].
AULT, GS ;
STONER, GL .
JOURNAL OF GENERAL VIROLOGY, 1993, 74 :1499-1507
[10]   Progressive multifocal leukoencephalopathy in patients with HIV infection [J].
Berger, JR ;
Pall, L ;
Lanska, D ;
Whiteman, M .
JOURNAL OF NEUROVIROLOGY, 1998, 4 (01) :59-68