Modification of hardwood dissolving pulp with purified Trichoderma reesei cellulases

被引:72
作者
Rahkamo, L
SiikaAho, M
Vehvilainen, M
Dolk, M
Viikari, L
Nousiainen, P
Buchert, J
机构
[1] TAMPERE UNIV TECHNOL,FIN-33101 TAMPERE,FINLAND
[2] KEMIRA FIBRES OY,FIN-37601 VALKEAKOSKI,FINLAND
[3] VTT BIOTECHNOL & FOOD RES,FIN-02044 ESPOO,ESPOO,FINLAND
关键词
cellulases; dissolving pulp; Trichoderma reesei; cellulose hydrolysis; alkaline solubility;
D O I
10.1007/BF02228798
中图分类号
TB3 [工程材料学]; TS [轻工业、手工业、生活服务业];
学科分类号
0805 ; 080502 ; 0822 ;
摘要
Hardwood dissolving pulp was treated with purified Trichoderma reesei endoglucanases and cellobiohydrolases. Endoglucanases were more efficient in hydrolysing pulp carbohydrates than were the cellobiohydrolases at the same protein dosage. Endoglucanases also lowered the viscosity and improved the alkaline solubility more dramatically. There was a clear correlation between the alkaline solubility and viscosity, and therefore the solubility could only be improved by lowering the viscosity of the pulp. At the same degree of cellulose degradation, endoglucanase II was found to be most effective in reducing the viscosity and thus improving the solubility. Cellobiohydrolases had a less pronounced effect on the viscosity or solubility.
引用
收藏
页码:153 / 163
页数:11
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