Structural characteristics of extra-membrane domains and guanidine hydrochloride-induced denaturation of photosystem 2 core antenna complexes CP43 and CP47

被引：7

作者：

Qu, Y. -G.

Gong, Y. -D.

Guo, S. -K.

Li, L. -B.

Kuang, T. -Y.

机构：

[1] Chinese Acad Sci, Inst Bot, Photosynth Res Ctr, Key Lab Photosynth & Environm Mol Physiol, Beijing 100093, Peoples R China

[2] Tsing Hua Univ, Dept Biol Sci & Biotechnol, State Key Lab Biomembrane & Membrane Biotechnol, Beijing 100084, Peoples R China

[3] Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China

来源：

PHOTOSYNTHETICA | 2006年 / 44卷 / 03期

基金：

中国国家自然科学基金;

关键词：

aromatic amino acid; chlorophyll; circular dichroism; CP43; CP47; denaturation; energy transfer; extra-membrane domain; fluorescence; guanidine hydrochloride; beta-carotene;

D O I：

10.1007/s11099-006-0049-y

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

The structural characteristics of the extra-membrane domains and guanidine hydrochloride-induced denaturation of photosystem 2 (PS2) core antenna complexes CP43 and CP47 were investigated using fluorescence emission and circular dichroism (CD) spectra. The extra-membrane domains of CP43 and CP47 possessed a certain degree of secondary and tertiary structure and not a complete random coil conformation. The tertiary structure and the chlorophyll (Chl) a microenvironment of CP47 were more sensitive to guanidine hydrochloride (GuHCl) than that of CP43: Changes in energy transfer from P-carotene to Chl a corresponded well to changes in the tertiary structure while their correlation with changes in the secondary structure was rather poor. Unlike most of water-soluble proteins, both CP43 and CP47 are partly resistant to denaturation induced by guanidine hydrochloride (GuHCl); the denaturation of CP43 or CP47 is not a two-state process. Those features most probably reflect their character as intrinsic membrane proteins.

引用

页码：447 / 453

页数：7

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