The Essential Oil Isolated from Artemisia capillaris Prevents LPS-Induced Production of NO and PGE2 by Inhibiting MAPK-Mediated Pathways in RAW 264.7 Macrophages

被引:24
作者
Cha, Jeong-Dan [3 ]
Moon, Sang-Eun [1 ,2 ]
Kim, Hye-Young [4 ]
Lee, Jeong-Chae [2 ,5 ,6 ]
Lee, Kyung-Yeol [1 ,2 ,6 ]
机构
[1] Chonbuk Natl Univ, Dept Oral Microbiol, Jeonju 561756, South Korea
[2] Chonbuk Natl Univ, Inst Oral Biosci, Jeonju 561756, South Korea
[3] Yonsei Univ, Coll Dent, Oral Canc Res Inst, Seoul 120749, South Korea
[4] Dongeul Univ, Dept Dent Hyg, Pusan, South Korea
[5] Chonbuk Natl Univ, Dept Orthodont, Lab Cell Biol, Jeonju 561756, South Korea
[6] Chonbuk Natl Univ, Res Ctr Bioact Mat, Jeonju 561756, South Korea
关键词
Artemisia capillaris; Essential oil; Lipopolysaccharide; Inflammation; MAPKs; Transcription factor; NF-KAPPA-B; INDUCED CYCLOOXYGENASE-2 EXPRESSION; SIGNAL-TRANSDUCTION PATHWAYS; NITRIC-OXIDE SYNTHASE; CHEMICAL-COMPOSITION; ACTIVATOR PROTEIN-1; OXIDATIVE STRESS; INOS EXPRESSION; GENE-EXPRESSION; RAW-264.7; CELLS;
D O I
10.1080/08820130902936307
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
071005 [微生物学]; 100108 [医学免疫学];
摘要
Artemisia capillaris (A. capillaris) is used in traditional Korean herbal medicine for its believedanti-inflammatory activities. Previous studies have suggested that the essential oil of A. capillaris contains the active components responsible for its pharmacological effect, even though the mechanism for its action is unclear. This study examined the inhibitory effects of the essential oil of A. capillaris on the lipopolysaccharide (LPS)-induced production of nitric oxide (NO) and prostaglandin E-2 (PGE(2)). The essential oil significantly inhibited the production of NO in the LPS-stimulated RAW 264.7 macrophages, which was mediated by the down-regulation of inducible NO synthase (iNOS) expression but not by its direct cytotoxic activity. The essential oil also blocked the secretion of PGE(2) and the expression of cyclooxygenase-2 (COX-2) in the LPS-stimulated cells. Western blot analysis showed that the essential oil inhibited the phosphorylation of I kappa B-alpha, nuclear translocation of p65, and subsequent activation of NF-kappa B. In addition, the essential oil suppressed the LPS-stimulated activation of mitogen-activated protein kinases (MAPKs) as well as the AP-1 DNA-binding activity. Moreover, MAPK inhibitors significantly reduced the LPS-induced production of NO and PGE(2). Collectively, we suggest that the oil inhibits the expression and production of inflammatory mediators by blocking the MAPK-mediated pathways and inhibiting the activation of NF-kappa B and AP-1.
引用
收藏
页码:783 / 497
页数:15
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