In situ hybridization study on tissue inhibitors of metalloproteinases (TIMPs) mRNA-expressing cells in human inflamed gingival tissue

被引：28

作者：

Kubota, T ^{[1
]}

Matsuki, Y ^{[1
]}

Nomura, T ^{[1
]}

Hara, K ^{[1
]}

机构：

[1] NIIGATA UNIV,SCH DENT,DEPT PERIODONTOL,NIIGATA 951,JAPAN

来源：

JOURNAL OF PERIODONTAL RESEARCH | 1997年 / 32卷 / 05期

关键词：

human gingiva; in situ hybridization; periodontal disease; tissue inhibitors of metalloproteinases (TIMPs);

D O I：

10.1111/j.1600-0765.1997.tb00559.x

中图分类号：

R78 [口腔科学];

学科分类号：

1003 ;

摘要：

This study presents the exact cell types and localization of tissue inhibitors of metalloproteinases (TIMPs) production sites in periodontal diseased gingiva by means of in situ hybridization. Gingival tissue specimens were fixed, embedded and hybridized in situ with specific digoxigenin-labeled cRNA probes (386 and 496 bp). TIMP-1 and -2 mRNAs were expressed on macrophages, mononuclear cells, capillary endothelial cells and some fibroblasts throughout the gingival tissue. In periodontitis, TIMP-1 and -2 mRNA-expressing cells showed significantly different localization. TIMP-1 mRNA was broadly observed in the gingival connective tissue while TIMP-2 mRNA was predominantly expressed in the connective tissue adjacent to the pocket epithelium (p < 0.01). Fewer TIMPs mRNA were observed in minimal gingivitis than in periodontitis, especially in the middle zone of gingival tissue. Thus, TIMP-1 and TIMP-2 mRNA was detected differentially and site-specifically in periodontal diseased gingival tissue.

引用

页码：467 / 472

页数：6

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