Isolation of a novel acidiphilic methanogen from an acidic peat bog

被引:128
作者
Braeuer, Suzanna L.
Cadillo-Quiroz, Hinsby
Yashiro, Erika
Yavitt, Joseph B.
Zinder, Stephen H. [1 ]
机构
[1] Cornell Univ, Dept Microbiol, Ithaca, NY 14853 USA
[2] Cornell Univ, Dept Nat Resources, Ithaca, NY 14853 USA
基金
美国国家科学基金会;
关键词
D O I
10.1038/nature04810
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Acidic peatlands are among the largest natural sources of atmospheric methane and harbour a large diversity of methanogenic Archaea(1). Despite the ubiquity of methanogens in these peatlands, indigenous methanogens capable of growth at acidic pH values have resisted culture and isolation(2-4); these recalcitrant methanogens include members of an uncultured family-level clade in the Methanomicrobiales prevalent in many acidic peat bogs in the Northern Hemisphere(1,5,6). However, we recently succeeded in obtaining a mixed enrichment culture of a member of this clade(7). Here we describe its isolation and initial characterization. We demonstrate that the optimum pH for methanogenesis by this organism is lower than that of any previously described methanogen.
引用
收藏
页码:192 / 194
页数:3
相关论文
共 25 条
[1]   Alternatives to binary fission in bacteria [J].
Angert, ER .
NATURE REVIEWS MICROBIOLOGY, 2005, 3 (03) :214-224
[2]   Methane biogeochemistry and methanogen communities in two northern peatland ecosystems, New York State [J].
Basiliko, N ;
Yavitt, JB ;
Dees, PM ;
Merkel, SM .
GEOMICROBIOLOGY JOURNAL, 2003, 20 (06) :563-577
[3]   Methanogenesis in McLean Bog, an acidic peat bog in upstate New York:: Stimulation by H2/CO2 in the presence of rifampicin, or by low concentrations of acetate [J].
Bräuer, SL ;
Yavitt, JB ;
Zinder, SH .
GEOMICROBIOLOGY JOURNAL, 2004, 21 (07) :433-443
[4]  
BRAUER SL, IN PRESS FEMS MICROB
[5]   Tiny bubbles tell all [J].
Brook, EJ .
SCIENCE, 2005, 310 (5752) :1285-1287
[6]  
CADILLOQUIROZ H, IN PRESS ENV MICROBI
[7]   BIOGEOCHEMICAL ASPECTS OF ATMOSPHERIC METHANE [J].
Cicerone, R. ;
Oremland, R. .
GLOBAL BIOGEOCHEMICAL CYCLES, 1988, 2 (04) :299-327
[8]   Isolation of acidophilic methane-oxidizing bacteria from northern peat wetlands [J].
Dedysh, SN ;
Panikov, NS ;
Liesack, W ;
Grosskopf, R ;
Zhou, JZ ;
Tiedje, JM .
SCIENCE, 1998, 282 (5387) :281-284
[9]   Methanogenium frigidum sp. nov., a psychrophilic, H-2-using methanogen from Ace Lake, Antarctica [J].
Franzmann, PD ;
Liu, YT ;
Balkwill, DL ;
Aldrich, HC ;
deMacario, EC ;
Boone, DR .
INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY, 1997, 47 (04) :1068-1072
[10]   Microsite-dependent changes in methanogenic populations in a boreal oligotrophic fen [J].
Galand, PE ;
Fritze, H ;
Yrjälä, K .
ENVIRONMENTAL MICROBIOLOGY, 2003, 5 (11) :1133-1143