Potent role of vaccines prepared from macrophages infected with live bacteria in protection against Mycobacterium tuberculosis and Salmonella typhimurium infections

被引：14

作者：

Sharma, N ^{[1
]}

Agrewala, JN ^{[1
]}

机构：

[1] Inst Microbiol Technol, Immunol Lab, Chandigarh 160036, India

来源：

JOURNAL OF INFECTIOUS DISEASES | 2004年 / 190卷 / 01期

关键词：

D O I：

10.1086/421116

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The present study describes a novel and simple vaccination strategy that involves the culturing of live Mycobacterium tuberculosis and Salmonella typhimurium in syngeneic, allogeneic, and xenogeneic macrophages, followed by drug treatment and gamma irradiation, to kill the bacteria. Notable observations were that the lymphocytes obtained from the vaccinated mice proliferated and secreted mainly interferon-gamma and IgG2a, but not interleukin-4 and IgG1. The enumeration of viability of M. tuberculosis indicated a significant level of protection in the vaccinated mice after challenge with live M. tuberculosis. This vaccination strategy worked successfully for tuberculosis but also showed a significant decrease in mortality of mice challenged with live S. typhimurium.

引用

页码：107 / 114

页数：8

共 43 条

[1]

Agrewala JN, 1998, J IMMUNOL, V160, P1067

[2] Delivery of antigen in allogeneic cells preferentially generates CD4+ Th1 cells [J].

Agrewala, JN ;

Suvas, S ;

Singh, V ;

Vohra, H .

CLINICAL AND EXPERIMENTAL IMMUNOLOGY, 2003, 134 (01) :13-22

[3]

Agrewala JN, 1999, EUR J IMMUNOL, V29, P1753, DOI 10.1002/(SICI)1521-4141(199906)29:06<1753::AID-IMMU1753>3.0.CO

[4]

2-B

[5]

Agrewala JN, 1998, CLIN EXP IMMUNOL, V114, P392

[6] Dendritic cells acquire antigen from apoptotic cells and induce class I restricted CTLs [J].

Albert, ML ;

Sauter, B ;

Bhardwaj, N .

NATURE, 1998, 392 (6671) :86-89

[7] CD4+ T-cell subsets that mediate immunological memory to Mycobacterium tuberculosis infection in mice [J].

Andersen, P ;

Smedegaard, B .

INFECTION AND IMMUNITY, 2000, 68 (02) :621-629

[8] EFFECTIVE VACCINATION OF MICE AGAINST MYCOBACTERIUM-TUBERCULOSIS INFECTION WITH A SOLUBLE MIXTURE OF SECRETED MYCOBACTERIAL PROTEINS [J].

ANDERSEN, P .

INFECTION AND IMMUNITY, 1994, 62 (06) :2536-2544

[9] PROTEINS RELEASED FROM MYCOBACTERIUM-TUBERCULOSIS DURING GROWTH [J].

ANDERSEN, P ;

ASKGAARD, D ;

LJUNGQVIST, L ;

BENNEDSEN, J ;

HERON, I .

INFECTION AND IMMUNITY, 1991, 59 (06) :1905-1910

[10]

Belz GT, 2002, CRIT REV IMMUNOL, V22, P439

← 1 2 3 4 5 →