Isolation of the gene coding for elongation factor-1 alpha in Cryptosporidium parvum

被引：19

作者：

Bonafonte, MT

Priest, JW

Garmon, D

Arrowood, MJ

Mead, JR

机构：

[1] EMORY UNIV,ATLANTA,GA 30033

[2] VET ADM MED CTR,DECATUR,GA 30033

[3] CTR DIS CONTROL & PREVENT,ATLANTA,GA 30341

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION | 1997年 / 1351卷 / 03期

关键词：

elongation factor 1 alpha; GTP-binding protein; (Cryptosporidium parvum);

D O I：

10.1016/S0167-4781(97)00013-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A gene encoding for Cryptosporidium parvum (C. parvum) elongation factor 1 alpha (EF-1 alpha) was isolated and sequenced from a cDNA expression library. The recombinant protein cross-reacted with a monoclonal antibody that was raised to a sporozoite cell surface antigen. The gene encoded a 435 amino acid protein with a predicted molecular weight of 48.1 kDa. The predicted C. parvum EF-1 alpha protein sequence showed extensive homology with the EF-1 alpha proteins of other eukaryotic organisms and included three conserved sequence motifs implicated in GTP binding. (C) 1997 Elsevier Science B.V.

引用

页码：256 / 260

页数：5

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