Localization and characteristics of DNA underreplication zone in the 75C region of intercalary heterochromatin in Drosophila melanogaster polytene chromosomes

被引：7

作者：

Andreyenkova, Natalya G. ^{[1
]}

Kokoza, Elena B. ^{[1
]}

Semeshin, Valery F. ^{[1
]}

Belyaeva, Elena S. ^{[1
]}

Demakov, Sergey A. ^{[1
]}

Pindyurin, Alexey V. ^{[1
]}

Andreyeva, Eugenia N. ^{[1
]}

Volkova, Elena I. ^{[1
]}

Zhimulev, Igor F. ^{[1
]}

机构：

[1] Russian Acad Sci, Dept Mol & Cellular Biol, Inst Chem Biol & Fundamental Med, Siberian Branch, Novosibirsk 630090, Russia

来源：

CHROMOSOMA | 2009年 / 118卷 / 06期

基金：

俄罗斯基础研究基金会;

关键词：

POSITION-EFFECT VARIEGATION; SUPPRESSOR-OF-UNDERREPLICATION; SUUR GENE; X-CHROMOSOME; REVISED MAP; CELL-TYPES; REPLICATION; MUTANTS; MINICHROMOSOME; ORGANIZATION;

D O I：

10.1007/s00412-009-0232-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

In Drosophila polytene chromosomes, regions of intercalary heterochromatin are scattered throughout the euchromatic arms. Here, we present data on the first fine analysis of the individual intercalary heterochromatin region, 75C1-2, located in the 3L chromosome. By using electron microscopy, we demonstrated that this region appears as three closely adjacent condensed bands. Mapping of the region on the physical map by means of the chromosomal rearrangements with known breakpoints showed that the length of the region is about 445 kb. Although it seems that the SUUR protein binds to the whole 75C1-2 region, the proximal part of the region is fully polytenized, so the DNA underreplication zone is asymmetric and located in the distal half of the region. Finally, we speculate that intercalary heterochromatin regions of Drosophila polytene chromosomes are organized into three different types with respect to the localization of the underreplication zone.

引用

页码：747 / 761

页数：15

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