Molecular cloning and biochemical characterization of a novel cytochrome P450, flavone synthase II, that catalyzes direct conversion of flavanones to flavones

被引：68

作者：

Akashi, T

Fukuchi-Mizutani, M

Aoki, T

Ueyama, Y

Yonekura-Sakakibara, K

Tanaka, Y

Kusumi, T

Ayabe, S ^{[1
]}

机构：

[1] Nihon Univ, Dept Appl Biol Sci, Fujisawa, Kanagawa 2528510, Japan

[2] Suntory Ltd, Inst Fundamental Res, Osaka 6188503, Japan

[3] Kobe Univ, Grad Sch Sci & Technol, Div Mol Sci, Kobe, Hyogo 6578501, Japan

来源：

PLANT AND CELL PHYSIOLOGY | 1999年 / 40卷 / 11期

关键词：

cDNA cloning; cytochrome P450; flavone synthase II; flavonoid biosynthesis; snapdragon; torenia;

D O I：

10.1093/oxfordjournals.pcp.a029505

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Cytochrome P450 cDNAs, AFNS2 and TFNS5, were isolated from snapdragon and torenia petal cDNA libraries, respectively, based on the sequence homology with Licorice CYP93B1 cDNA encoding (2S)-flavanone 2-hydroxylase. They were expressed in yeast and identified to encode flavone synthase II catalyzing direct conversion of flavanones to flavones probably via 2-hydroxyflavanones.

引用

页码：1182 / 1186

页数：5

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