Laccase immobilization in redox active layered double hydroxides: A reagentless amperometric biosensor

被引：82

作者：

Mousty, Christine ^{[1
]}

Vieille, Laetitia ^{[1
]}

Cosnier, Serge ^{[1
]}

机构：

[1] Univ Grenoble 1, CNRS, FR CNRS 2607,Inst Chim Mol Grenoble, UMR 5630,Lab Electrochim Organ & Photochim Redox, F-38042 Grenoble, France

来源：

BIOSENSORS & BIOELECTRONICS | 2007年 / 22卷 / 08期

关键词：

laccase; layered double hydroxides; anionic clay; biosensor; O-2; reduction; inhibition;

D O I：

10.1016/j.bios.2006.08.020

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

This paper describes a new system for amperometric determination of dissolved oxygen and its application for the detection of anionic toxic substances, which are known as enzyme inhibitors. This biosensor is based on the co-immobilization of laccase from Trametes versicolor and a redox active layered double hydroxide [Zn-Cr-ABTS] on a glassy carbon electrode. The electrochemical transduction step corresponds to the electrocatalytic reduction Of O-2 at 0.2 V by laccase as catalyst and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) as mediator. Such device provides a fast and a sensitive response for dissolved oxygen determination between 6 x 10(-8) and 4 x 10(-6) M and very low detection limits for azide (5.5 nM), fluoride (6.9 nM) and cyanide (6.2 nM). (c) 2006 Elsevier B.V. All rights reserved.

引用

页码：1733 / 1738

页数：6

共 40 条

[1] Electroreduction of O2 to water on the "Wired" laccase cathode [J].