In Vivo Analysis of the Notch Receptor S1 Cleavage

被引：41

作者：

Lake, Robert J.

Grimm, Lisa M.

Veraksa, Alexey

Banos, Andrew

Artavanis-Tsakonas, Spyros

机构：

[1] Department of Cell Biology, Harvard Medical School, Boston, MA

[2] Biology Department, University of Massachusetts Boston, Boston, MA

[3] Collège de France, Paris

[4] Epigenetics and Progenitor Cell Program, Fox Chase Cancer Center, Philadelphia, PA

来源：

PLOS ONE | 2009年 / 4卷 / 08期

关键词：

PROTEOLYTIC RELEASE; DROSOPHILA; DELTA; EXPRESSION;

D O I：

10.1371/journal.pone.0006728

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

070301 [无机化学]; 070403 [天体物理学]; 070507 [自然资源与国土空间规划学]; 090105 [作物生产系统与生态工程];

摘要：

A ligand-independent cleavage (S1) in the extracellular domain of the mammalian Notch receptor results in what is considered to be the canonical heterodimeric form of Notch on the cell surface. The in vivo consequences and significance of this cleavage on Drosophila Notch signaling remain unclear and contradictory. We determined the cleavage site in Drosophila and examined its in vivo function by a transgenic analysis of receptors that cannot be cleaved. Our results demonstrate a correlation between loss of cleavage and loss of in vivo function of the Notch receptor, supporting the notion that S1 cleavage is an in vivo mechanism of Notch signal control.

引用

页数：9

共 31 条

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