Dbp10p, a putative RNA helicase from Saccharomyces cerevisiae, is required for ribosome biogenesis

被引:48
作者
Burger, F [1 ]
Daugeron, MC [1 ]
Linder, P [1 ]
机构
[1] CMU, Dept Biochim Med, CH-1211 Geneva 4, Switzerland
关键词
D O I
10.1093/nar/28.12.2315
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Ribosome biogenesis requires, in addition to rRNA molecules and ribosomal proteins, a multitude of trans-acting factors. Recently it has become clear that in the yeast Saccharomyces cerevisiae many RNA helicases of the DEAD-box and related families are involved in ribosome biogenesis, Here we show that the previously uncharacterised open reading frame YDL031w (renamed DBP10 for (D) under bar EAD-(b) under bar ox (p) under bar rotein <(10)under bar>) encodes an essential putative RNA helicase that is required for accurate ribosome biogenesis, Genetic depletion of Dbp10p results in a deficit in 60S ribosomal subunits and an accumulation of half-mer polysomes. Furthermore, pulse-chase analyses of pre-rRNA processing reveal a strong delay in the maturation of 27SB pre-rRNA intermediates into 25S rRNA and 7S pre-rRNA. Northern blot analyses indicate that this delay leads to higher steady-state levels of 27SB species and reduced steady-state levels of 7S pre-rRNA and 25S/5.8S mature rRNAs, thus explaining the final deficit in 60S subunit and the formation of half-mer polysomes. Consistent with a direct role in ribosome biogenesis, Dbp10p was found to be located predominantly in the nucleolus.
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页码:2315 / 2323
页数:9
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