Syntheses and spectral properties of fluorescent trimethine sulfo-3H-indocyanine dyes

被引:26
作者
Wang, LQ
Peng, XJ [1 ]
Zhang, R
Cui, JN
Xu, GQ
Wang, FG
机构
[1] Dalian Univ Technol, State Key Lab Fine Chem, Dalian 116012, Peoples R China
[2] Quqihar Univ, Qiqihar 161006, Peoples R China
[3] Lines Technol Corp, Raleigh, NC 27612 USA
基金
中国国家自然科学基金;
关键词
sulfo-3H-indocyanine; fluorescent dyes; biological label;
D O I
10.1016/S0143-7208(02)00036-0
中图分类号
O69 [应用化学];
学科分类号
081704 ;
摘要
Novel fluorescent sulfo-3H-indocyanine dyes containing at least one p-carboxybenzyl group on the nitrogen atoms in 3H-indocyanine rings were synthesized. Their lambda(abs) are in the range of 547-551 nm and lambda(em) 562-567 nm in water. When solvents change from water to ethanol or DMF, the lambda(abs) and lambda(em) of the dyes exhibit red shifts. Because of the presence of sulfonate groups and carboxyl groups, these dyes have good aqueous solubility. The p-carboxybenzyl group let the dyes have better photo-stability than commercial 5-carboxylpentynyl- containing dyes. The carboxyl groups can be converted into their N-hydroxylsuccinimidyl esters to label proteins, oligonucleotides or other bio-chemicals. (C) 2002 Elsevier Science Ltd. All rights reserved.
引用
收藏
页码:107 / 111
页数:5
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