In vivo flow cytometry:: A new method for enumerating circulating cancer cells

被引：160

作者：

Georgakoudi, I ^{[1
]}

Solban, N ^{[1
]}

Novak, J ^{[1
]}

Rice, WL ^{[1
]}

Wei, XB ^{[1
]}

Hasan, T ^{[1
]}

Lin, CP ^{[1
]}

机构：

[1] Harvard Univ, Massachusetts Gen Hosp, Sch Med, Wellman Ctr Photomed, Boston, MA 02114 USA

来源：

CANCER RESEARCH | 2004年 / 64卷 / 15期

关键词：

D O I：

10.1158/0008-5472.CAN-04-1058

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

The fate of circulating tumor cells is an important determinant of their ability to form distant metastasis. Here, we demonstrate the use of in VIVO flow cytometry as a powerful new method for detecting quantitatively circulating cancer cells. We specifically examine the circulation kinetics of two prostate cancer cell lines with different metastatic potential in mice and rats. We find that the cell line and the host environment affect the circulation kinetics of prostate cancer cells, with the intrinsic cell line properties determining the initial rate of cell depletion from the circulation and the host affecting cell circulation at later time points.

引用

页码：5044 / 5047

页数：4

共 19 条

[1] Intravascular origin of metastasis from the proliferation of endothelium-attached tumor cells: a new model for metastasis [J].

Al-Mehdi, AB ;

Tozawa, K ;

Fisher, AB ;

Shientag, L ;

Lee, A ;

Muschel, RJ .

NATURE MEDICINE, 2000, 6 (01) :100-102

[2] Noninvasive imaging of in vivo blood flow velocity using optical Doppler tomography [J].

Chen, ZP ;

Milner, TE ;

Srinivas, S ;

Wang, XJ ;

Malekafzali, A ;

vanGemert, MJC ;

Nelson, JS .

OPTICS LETTERS, 1997, 22 (14) :1119-1121

[3] In vivo evaluation of the early events associated with liver metastasis of circulating cancer cells [J].

Ding, L ;

Sunamura, M ;

Kodama, T ;

Yamauchi, J ;

Duda, DG ;

Shimamura, H ;

Shibuya, K ;

Takeda, K ;

Matsuno, S .

BRITISH JOURNAL OF CANCER, 2001, 85 (03) :431-438

[4] Detection of single mammalian cells by high-resolution magnetic resonance imaging [J].

Dodd, SJ ;

Williams, M ;

Suhan, JP ;

Williams, DS ;

Koretsky, AP ;

Ho, C .

BIOPHYSICAL JOURNAL, 1999, 76 (01) :103-109

[5] Quantitative imaging of the T cell antitumor response by positron-emission tomography [J].

Dubey, P ;

Su, H ;

Adonai, N ;

Du, SY ;

Rosato, A ;

Braun, J ;

Gambhir, SS ;

Witte, ON .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2003, 100 (03) :1232-1237

[6]

FIDLER IJ, 1970, JNCI-J NATL CANCER I, V45, P773

[7] Timeline - The pathogenesis of cancer metastasis: the 'seed and soil' hypothesis revisited [J].

Fidler, IJ .

NATURE REVIEWS CANCER, 2003, 3 (06) :453-458

[8] Laser-induced hyperthermia in rat prostate cancer: Role of site of tumor implantation [J].

Lein, M ;

Koenig, F ;

Misdraji, J ;

McDougal, WS ;

Jung, K ;

Loening, SA ;

Hasan, T ;

Ortel, B .

UROLOGY, 2000, 56 (01) :167-172

[9]

Momma T, 1998, CANCER RES, V58, P5425

[10]

Naumov GN, 1999, J CELL SCI, V112, P1835

← 1 2 →